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Recent advances in proximity-based labeling methods for interactome mapping

Proximity-based labeling has emerged as a powerful complementary approach to classic affinity purification of multiprotein complexes in the mapping of protein–protein interactions. Ongoing optimization of enzyme tags and delivery methods has improved both temporal and spatial resolution, and the tec...

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Autor principal: Trinkle-Mulcahy, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357996/
https://www.ncbi.nlm.nih.gov/pubmed/30774936
http://dx.doi.org/10.12688/f1000research.16903.1
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author Trinkle-Mulcahy, Laura
author_facet Trinkle-Mulcahy, Laura
author_sort Trinkle-Mulcahy, Laura
collection PubMed
description Proximity-based labeling has emerged as a powerful complementary approach to classic affinity purification of multiprotein complexes in the mapping of protein–protein interactions. Ongoing optimization of enzyme tags and delivery methods has improved both temporal and spatial resolution, and the technique has been successfully employed in numerous small-scale (single complex mapping) and large-scale (network mapping) initiatives. When paired with quantitative proteomic approaches, the ability of these assays to provide snapshots of stable and transient interactions over time greatly facilitates the mapping of dynamic interactomes. Furthermore, recent innovations have extended biotin-based proximity labeling techniques such as BioID and APEX beyond classic protein-centric assays (tag a protein to label neighboring proteins) to include RNA-centric (tag an RNA species to label RNA-binding proteins) and DNA-centric (tag a gene locus to label associated protein complexes) assays.
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spelling pubmed-63579962019-02-14 Recent advances in proximity-based labeling methods for interactome mapping Trinkle-Mulcahy, Laura F1000Res Review Proximity-based labeling has emerged as a powerful complementary approach to classic affinity purification of multiprotein complexes in the mapping of protein–protein interactions. Ongoing optimization of enzyme tags and delivery methods has improved both temporal and spatial resolution, and the technique has been successfully employed in numerous small-scale (single complex mapping) and large-scale (network mapping) initiatives. When paired with quantitative proteomic approaches, the ability of these assays to provide snapshots of stable and transient interactions over time greatly facilitates the mapping of dynamic interactomes. Furthermore, recent innovations have extended biotin-based proximity labeling techniques such as BioID and APEX beyond classic protein-centric assays (tag a protein to label neighboring proteins) to include RNA-centric (tag an RNA species to label RNA-binding proteins) and DNA-centric (tag a gene locus to label associated protein complexes) assays. F1000 Research Limited 2019-01-31 /pmc/articles/PMC6357996/ /pubmed/30774936 http://dx.doi.org/10.12688/f1000research.16903.1 Text en Copyright: © 2019 Trinkle-Mulcahy L http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Trinkle-Mulcahy, Laura
Recent advances in proximity-based labeling methods for interactome mapping
title Recent advances in proximity-based labeling methods for interactome mapping
title_full Recent advances in proximity-based labeling methods for interactome mapping
title_fullStr Recent advances in proximity-based labeling methods for interactome mapping
title_full_unstemmed Recent advances in proximity-based labeling methods for interactome mapping
title_short Recent advances in proximity-based labeling methods for interactome mapping
title_sort recent advances in proximity-based labeling methods for interactome mapping
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357996/
https://www.ncbi.nlm.nih.gov/pubmed/30774936
http://dx.doi.org/10.12688/f1000research.16903.1
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