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Recent advances in proximity-based labeling methods for interactome mapping
Proximity-based labeling has emerged as a powerful complementary approach to classic affinity purification of multiprotein complexes in the mapping of protein–protein interactions. Ongoing optimization of enzyme tags and delivery methods has improved both temporal and spatial resolution, and the tec...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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F1000 Research Limited
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357996/ https://www.ncbi.nlm.nih.gov/pubmed/30774936 http://dx.doi.org/10.12688/f1000research.16903.1 |
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author | Trinkle-Mulcahy, Laura |
author_facet | Trinkle-Mulcahy, Laura |
author_sort | Trinkle-Mulcahy, Laura |
collection | PubMed |
description | Proximity-based labeling has emerged as a powerful complementary approach to classic affinity purification of multiprotein complexes in the mapping of protein–protein interactions. Ongoing optimization of enzyme tags and delivery methods has improved both temporal and spatial resolution, and the technique has been successfully employed in numerous small-scale (single complex mapping) and large-scale (network mapping) initiatives. When paired with quantitative proteomic approaches, the ability of these assays to provide snapshots of stable and transient interactions over time greatly facilitates the mapping of dynamic interactomes. Furthermore, recent innovations have extended biotin-based proximity labeling techniques such as BioID and APEX beyond classic protein-centric assays (tag a protein to label neighboring proteins) to include RNA-centric (tag an RNA species to label RNA-binding proteins) and DNA-centric (tag a gene locus to label associated protein complexes) assays. |
format | Online Article Text |
id | pubmed-6357996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-63579962019-02-14 Recent advances in proximity-based labeling methods for interactome mapping Trinkle-Mulcahy, Laura F1000Res Review Proximity-based labeling has emerged as a powerful complementary approach to classic affinity purification of multiprotein complexes in the mapping of protein–protein interactions. Ongoing optimization of enzyme tags and delivery methods has improved both temporal and spatial resolution, and the technique has been successfully employed in numerous small-scale (single complex mapping) and large-scale (network mapping) initiatives. When paired with quantitative proteomic approaches, the ability of these assays to provide snapshots of stable and transient interactions over time greatly facilitates the mapping of dynamic interactomes. Furthermore, recent innovations have extended biotin-based proximity labeling techniques such as BioID and APEX beyond classic protein-centric assays (tag a protein to label neighboring proteins) to include RNA-centric (tag an RNA species to label RNA-binding proteins) and DNA-centric (tag a gene locus to label associated protein complexes) assays. F1000 Research Limited 2019-01-31 /pmc/articles/PMC6357996/ /pubmed/30774936 http://dx.doi.org/10.12688/f1000research.16903.1 Text en Copyright: © 2019 Trinkle-Mulcahy L http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Trinkle-Mulcahy, Laura Recent advances in proximity-based labeling methods for interactome mapping |
title | Recent advances in proximity-based labeling methods for interactome mapping |
title_full | Recent advances in proximity-based labeling methods for interactome mapping |
title_fullStr | Recent advances in proximity-based labeling methods for interactome mapping |
title_full_unstemmed | Recent advances in proximity-based labeling methods for interactome mapping |
title_short | Recent advances in proximity-based labeling methods for interactome mapping |
title_sort | recent advances in proximity-based labeling methods for interactome mapping |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357996/ https://www.ncbi.nlm.nih.gov/pubmed/30774936 http://dx.doi.org/10.12688/f1000research.16903.1 |
work_keys_str_mv | AT trinklemulcahylaura recentadvancesinproximitybasedlabelingmethodsforinteractomemapping |