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Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization
Cell-free synthetic biology is an exciting and new branch in the field of synthetic biology. Based on in vitro transcription and translation systems, this application-focused domain builds on decades of cell-free biochemistry and protein expression to operate synthetic gene networks outside of cellu...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Elsevier
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358126/ https://www.ncbi.nlm.nih.gov/pubmed/30740032 http://dx.doi.org/10.1016/j.bej.2018.07.008 |
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author | Pardee, Keith |
author_facet | Pardee, Keith |
author_sort | Pardee, Keith |
collection | PubMed |
description | Cell-free synthetic biology is an exciting and new branch in the field of synthetic biology. Based on in vitro transcription and translation systems, this application-focused domain builds on decades of cell-free biochemistry and protein expression to operate synthetic gene networks outside of cellular environments. This has brought new and perhaps even unexpected advantages. Chief among these is the ability to operate genetically encoded tools in a sterile and abiotic format. Recent work has extended this advantage by freeze-drying these cell-free systems into dried pellets or embedded paper-based reactions. Taken together, these new ideas have solved the longstanding challenge of how to deploy poised synthetic gene networks in a biosafe mode outside of the laboratory. There is significant excitement in the potential of this newfound venue and the community has begun to extend proof-of-concept demonstrations in important and creative ways. Here I explore these new efforts and provide my thoughts on the challenges and opportunities ahead for freeze-dried, cell-free synthetic biology. |
format | Online Article Text |
id | pubmed-6358126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-63581262019-02-08 Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization Pardee, Keith Biochem Eng J Article Cell-free synthetic biology is an exciting and new branch in the field of synthetic biology. Based on in vitro transcription and translation systems, this application-focused domain builds on decades of cell-free biochemistry and protein expression to operate synthetic gene networks outside of cellular environments. This has brought new and perhaps even unexpected advantages. Chief among these is the ability to operate genetically encoded tools in a sterile and abiotic format. Recent work has extended this advantage by freeze-drying these cell-free systems into dried pellets or embedded paper-based reactions. Taken together, these new ideas have solved the longstanding challenge of how to deploy poised synthetic gene networks in a biosafe mode outside of the laboratory. There is significant excitement in the potential of this newfound venue and the community has begun to extend proof-of-concept demonstrations in important and creative ways. Here I explore these new efforts and provide my thoughts on the challenges and opportunities ahead for freeze-dried, cell-free synthetic biology. Elsevier 2018-10-15 /pmc/articles/PMC6358126/ /pubmed/30740032 http://dx.doi.org/10.1016/j.bej.2018.07.008 Text en © 2018 The Author http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Pardee, Keith Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization |
title | Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization |
title_full | Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization |
title_fullStr | Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization |
title_full_unstemmed | Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization |
title_short | Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization |
title_sort | perspective: solidifying the impact of cell-free synthetic biology through lyophilization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358126/ https://www.ncbi.nlm.nih.gov/pubmed/30740032 http://dx.doi.org/10.1016/j.bej.2018.07.008 |
work_keys_str_mv | AT pardeekeith perspectivesolidifyingtheimpactofcellfreesyntheticbiologythroughlyophilization |