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Learning the space-time phase diagram of bacterial swarm expansion
Coordinated dynamics of individual components in active matter are an essential aspect of life on all scales. Establishing a comprehensive, causal connection between intracellular, intercellular, and macroscopic behaviors has remained a major challenge due to limitations in data acquisition and anal...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358709/ https://www.ncbi.nlm.nih.gov/pubmed/30635422 http://dx.doi.org/10.1073/pnas.1811722116 |
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author | Jeckel, Hannah Jelli, Eric Hartmann, Raimo Singh, Praveen K. Mok, Rachel Totz, Jan Frederik Vidakovic, Lucia Eckhardt, Bruno Dunkel, Jörn Drescher, Knut |
author_facet | Jeckel, Hannah Jelli, Eric Hartmann, Raimo Singh, Praveen K. Mok, Rachel Totz, Jan Frederik Vidakovic, Lucia Eckhardt, Bruno Dunkel, Jörn Drescher, Knut |
author_sort | Jeckel, Hannah |
collection | PubMed |
description | Coordinated dynamics of individual components in active matter are an essential aspect of life on all scales. Establishing a comprehensive, causal connection between intracellular, intercellular, and macroscopic behaviors has remained a major challenge due to limitations in data acquisition and analysis techniques suitable for multiscale dynamics. Here, we combine a high-throughput adaptive microscopy approach with machine learning, to identify key biological and physical mechanisms that determine distinct microscopic and macroscopic collective behavior phases which develop as Bacillus subtilis swarms expand over five orders of magnitude in space. Our experiments, continuum modeling, and particle-based simulations reveal that macroscopic swarm expansion is primarily driven by cellular growth kinetics, whereas the microscopic swarming motility phases are dominated by physical cell–cell interactions. These results provide a unified understanding of bacterial multiscale behavioral complexity in swarms. |
format | Online Article Text |
id | pubmed-6358709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-63587092019-02-05 Learning the space-time phase diagram of bacterial swarm expansion Jeckel, Hannah Jelli, Eric Hartmann, Raimo Singh, Praveen K. Mok, Rachel Totz, Jan Frederik Vidakovic, Lucia Eckhardt, Bruno Dunkel, Jörn Drescher, Knut Proc Natl Acad Sci U S A Physical Sciences Coordinated dynamics of individual components in active matter are an essential aspect of life on all scales. Establishing a comprehensive, causal connection between intracellular, intercellular, and macroscopic behaviors has remained a major challenge due to limitations in data acquisition and analysis techniques suitable for multiscale dynamics. Here, we combine a high-throughput adaptive microscopy approach with machine learning, to identify key biological and physical mechanisms that determine distinct microscopic and macroscopic collective behavior phases which develop as Bacillus subtilis swarms expand over five orders of magnitude in space. Our experiments, continuum modeling, and particle-based simulations reveal that macroscopic swarm expansion is primarily driven by cellular growth kinetics, whereas the microscopic swarming motility phases are dominated by physical cell–cell interactions. These results provide a unified understanding of bacterial multiscale behavioral complexity in swarms. National Academy of Sciences 2019-01-29 2019-01-11 /pmc/articles/PMC6358709/ /pubmed/30635422 http://dx.doi.org/10.1073/pnas.1811722116 Text en Copyright © 2019 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Physical Sciences Jeckel, Hannah Jelli, Eric Hartmann, Raimo Singh, Praveen K. Mok, Rachel Totz, Jan Frederik Vidakovic, Lucia Eckhardt, Bruno Dunkel, Jörn Drescher, Knut Learning the space-time phase diagram of bacterial swarm expansion |
title | Learning the space-time phase diagram of bacterial swarm expansion |
title_full | Learning the space-time phase diagram of bacterial swarm expansion |
title_fullStr | Learning the space-time phase diagram of bacterial swarm expansion |
title_full_unstemmed | Learning the space-time phase diagram of bacterial swarm expansion |
title_short | Learning the space-time phase diagram of bacterial swarm expansion |
title_sort | learning the space-time phase diagram of bacterial swarm expansion |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358709/ https://www.ncbi.nlm.nih.gov/pubmed/30635422 http://dx.doi.org/10.1073/pnas.1811722116 |
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