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THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis

To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also st...

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Autores principales: Khan, Zaeema, Karamahmutoğlu, Hande, Elitaş, Meltem, Yüce, Meral, Budak, Hikmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358813/
https://www.ncbi.nlm.nih.gov/pubmed/30625995
http://dx.doi.org/10.3390/plants8010014
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author Khan, Zaeema
Karamahmutoğlu, Hande
Elitaş, Meltem
Yüce, Meral
Budak, Hikmet
author_facet Khan, Zaeema
Karamahmutoğlu, Hande
Elitaş, Meltem
Yüce, Meral
Budak, Hikmet
author_sort Khan, Zaeema
collection PubMed
description To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.
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spelling pubmed-63588132019-02-11 THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis Khan, Zaeema Karamahmutoğlu, Hande Elitaş, Meltem Yüce, Meral Budak, Hikmet Plants (Basel) Article To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress. MDPI 2019-01-08 /pmc/articles/PMC6358813/ /pubmed/30625995 http://dx.doi.org/10.3390/plants8010014 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khan, Zaeema
Karamahmutoğlu, Hande
Elitaş, Meltem
Yüce, Meral
Budak, Hikmet
THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
title THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
title_full THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
title_fullStr THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
title_full_unstemmed THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
title_short THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
title_sort through the looking glass: real-time imaging in brachypodium roots and osmotic stress analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358813/
https://www.ncbi.nlm.nih.gov/pubmed/30625995
http://dx.doi.org/10.3390/plants8010014
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