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Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3)

Magnesium (Mg) is crucial for bone health. Low concentrations of Mg inhibit the activity of osteoblasts while promoting that of osteoclasts, with the final result of inducing osteopenia. Conversely, little is known about the effects of high concentrations of extracellular Mg on osteoclasts and osteo...

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Autores principales: Mammoli, Fabiana, Castiglioni, Sara, Parenti, Sandra, Cappadone, Concettina, Farruggia, Giovanna, Iotti, Stefano, Davalli, Pierpaola, Maier, Jeanette A.M., Grande, Alexis, Frassineti, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358963/
https://www.ncbi.nlm.nih.gov/pubmed/30658432
http://dx.doi.org/10.3390/ijms20020385
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author Mammoli, Fabiana
Castiglioni, Sara
Parenti, Sandra
Cappadone, Concettina
Farruggia, Giovanna
Iotti, Stefano
Davalli, Pierpaola
Maier, Jeanette A.M.
Grande, Alexis
Frassineti, Chiara
author_facet Mammoli, Fabiana
Castiglioni, Sara
Parenti, Sandra
Cappadone, Concettina
Farruggia, Giovanna
Iotti, Stefano
Davalli, Pierpaola
Maier, Jeanette A.M.
Grande, Alexis
Frassineti, Chiara
author_sort Mammoli, Fabiana
collection PubMed
description Magnesium (Mg) is crucial for bone health. Low concentrations of Mg inhibit the activity of osteoblasts while promoting that of osteoclasts, with the final result of inducing osteopenia. Conversely, little is known about the effects of high concentrations of extracellular Mg on osteoclasts and osteoblasts. Since the differentiation and activation of these cells is coordinated by vitamin D(3) (VD3), we investigated the effects of high extracellular Mg, as well as its impact on VD3 activity, in these cells. U937 cells were induced to osteoclastic differentiation by VD3 in the presence of supra-physiological concentrations (>1 mM) of extracellular Mg. The effect of high Mg concentrations was also studied in human bone-marrow-derived mesenchymal stem cells (bMSCs) induced to differentiate into osteoblasts by VD3. We demonstrate that high extra-cellular Mg levels potentiate VD3-induced osteoclastic differentiation, while decreasing osteoblastogenesis. We hypothesize that Mg might reprogram VD3 activity on bone remodeling, causing an unbalanced activation of osteoclasts and osteoblasts.
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spelling pubmed-63589632019-02-06 Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3) Mammoli, Fabiana Castiglioni, Sara Parenti, Sandra Cappadone, Concettina Farruggia, Giovanna Iotti, Stefano Davalli, Pierpaola Maier, Jeanette A.M. Grande, Alexis Frassineti, Chiara Int J Mol Sci Article Magnesium (Mg) is crucial for bone health. Low concentrations of Mg inhibit the activity of osteoblasts while promoting that of osteoclasts, with the final result of inducing osteopenia. Conversely, little is known about the effects of high concentrations of extracellular Mg on osteoclasts and osteoblasts. Since the differentiation and activation of these cells is coordinated by vitamin D(3) (VD3), we investigated the effects of high extracellular Mg, as well as its impact on VD3 activity, in these cells. U937 cells were induced to osteoclastic differentiation by VD3 in the presence of supra-physiological concentrations (>1 mM) of extracellular Mg. The effect of high Mg concentrations was also studied in human bone-marrow-derived mesenchymal stem cells (bMSCs) induced to differentiate into osteoblasts by VD3. We demonstrate that high extra-cellular Mg levels potentiate VD3-induced osteoclastic differentiation, while decreasing osteoblastogenesis. We hypothesize that Mg might reprogram VD3 activity on bone remodeling, causing an unbalanced activation of osteoclasts and osteoblasts. MDPI 2019-01-17 /pmc/articles/PMC6358963/ /pubmed/30658432 http://dx.doi.org/10.3390/ijms20020385 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mammoli, Fabiana
Castiglioni, Sara
Parenti, Sandra
Cappadone, Concettina
Farruggia, Giovanna
Iotti, Stefano
Davalli, Pierpaola
Maier, Jeanette A.M.
Grande, Alexis
Frassineti, Chiara
Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3)
title Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3)
title_full Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3)
title_fullStr Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3)
title_full_unstemmed Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3)
title_short Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D(3)
title_sort magnesium is a key regulator of the balance between osteoclast and osteoblast differentiation in the presence of vitamin d(3)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358963/
https://www.ncbi.nlm.nih.gov/pubmed/30658432
http://dx.doi.org/10.3390/ijms20020385
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