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Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model

The use of chitosan nanoparticles (ChNPs) in various biological and environmental applications is attracting great interest. However, potential side effects related to ChNP toxicity remain the major limitation hampering their wide application. For the first time, we investigate the potential organ-s...

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Autores principales: Abou-Saleh, Haissam, Younes, Nadin, Rasool, Kashif, Younis, Manaf H., Prieto, Rafael M., Yassine, Hadi M., Mahmoud, Khaled A., Pintus, Gianfranco, Nasrallah, Gheyath K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359003/
https://www.ncbi.nlm.nih.gov/pubmed/30669437
http://dx.doi.org/10.3390/nano9010122
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author Abou-Saleh, Haissam
Younes, Nadin
Rasool, Kashif
Younis, Manaf H.
Prieto, Rafael M.
Yassine, Hadi M.
Mahmoud, Khaled A.
Pintus, Gianfranco
Nasrallah, Gheyath K.
author_facet Abou-Saleh, Haissam
Younes, Nadin
Rasool, Kashif
Younis, Manaf H.
Prieto, Rafael M.
Yassine, Hadi M.
Mahmoud, Khaled A.
Pintus, Gianfranco
Nasrallah, Gheyath K.
author_sort Abou-Saleh, Haissam
collection PubMed
description The use of chitosan nanoparticles (ChNPs) in various biological and environmental applications is attracting great interest. However, potential side effects related to ChNP toxicity remain the major limitation hampering their wide application. For the first time, we investigate the potential organ-specific (cardiac, hepatic, and neuromuscular) toxicity of ChNPs (size 100–150 nm) using the zebrafish embryo model. Our data highlight the absence of both acute and teratogenic toxic effects of ChNPs (~100% survival rate) even at the higher concentration employed (200 mg/L). Although no single sign of cardiotoxicity was observed upon exposure to 200 mg/L of ChNPs, as judged by heartbeat rate, the corrected QT interval (QTc, which measures the time between the start of the Q wave and the end of the T wave in the heart's electrical cycle), maximum cardiac arrest, and ejection fraction assays, the same dosage elicited the impairment of both liver size (decreased liver size, but without steatosis and lipid yolk retention) and neurobehavioral activity (increased movement under different light conditions). Although the observed toxic effect failed to affect embryo survival, whether a prolonged ChNP treatment may induce other potentially harmful effects remains to be elucidated. By reporting new insights on their organ-specific toxicity, our results add novel and useful information into the available data concerning the in vivo effect of ChNPs.
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spelling pubmed-63590032019-02-06 Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model Abou-Saleh, Haissam Younes, Nadin Rasool, Kashif Younis, Manaf H. Prieto, Rafael M. Yassine, Hadi M. Mahmoud, Khaled A. Pintus, Gianfranco Nasrallah, Gheyath K. Nanomaterials (Basel) Article The use of chitosan nanoparticles (ChNPs) in various biological and environmental applications is attracting great interest. However, potential side effects related to ChNP toxicity remain the major limitation hampering their wide application. For the first time, we investigate the potential organ-specific (cardiac, hepatic, and neuromuscular) toxicity of ChNPs (size 100–150 nm) using the zebrafish embryo model. Our data highlight the absence of both acute and teratogenic toxic effects of ChNPs (~100% survival rate) even at the higher concentration employed (200 mg/L). Although no single sign of cardiotoxicity was observed upon exposure to 200 mg/L of ChNPs, as judged by heartbeat rate, the corrected QT interval (QTc, which measures the time between the start of the Q wave and the end of the T wave in the heart's electrical cycle), maximum cardiac arrest, and ejection fraction assays, the same dosage elicited the impairment of both liver size (decreased liver size, but without steatosis and lipid yolk retention) and neurobehavioral activity (increased movement under different light conditions). Although the observed toxic effect failed to affect embryo survival, whether a prolonged ChNP treatment may induce other potentially harmful effects remains to be elucidated. By reporting new insights on their organ-specific toxicity, our results add novel and useful information into the available data concerning the in vivo effect of ChNPs. MDPI 2019-01-19 /pmc/articles/PMC6359003/ /pubmed/30669437 http://dx.doi.org/10.3390/nano9010122 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abou-Saleh, Haissam
Younes, Nadin
Rasool, Kashif
Younis, Manaf H.
Prieto, Rafael M.
Yassine, Hadi M.
Mahmoud, Khaled A.
Pintus, Gianfranco
Nasrallah, Gheyath K.
Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model
title Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model
title_full Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model
title_fullStr Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model
title_full_unstemmed Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model
title_short Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model
title_sort impaired liver size and compromised neurobehavioral activity are elicited by chitosan nanoparticles in the zebrafish embryo model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359003/
https://www.ncbi.nlm.nih.gov/pubmed/30669437
http://dx.doi.org/10.3390/nano9010122
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