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HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight

Heat Shock Factor A2 (HsfA2) is part of the Heat Shock Factor (HSF) network, and plays an essential role beyond heat shock in environmental stress responses and cellular homeostatic control. Arabidopsis thaliana cell cultures derived from wild type (WT) ecotype Col-0 and a knockout line deficient in...

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Autores principales: Zupanska, Agata K., LeFrois, Collin, Ferl, Robert J., Paul, Anna-Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359015/
https://www.ncbi.nlm.nih.gov/pubmed/30658467
http://dx.doi.org/10.3390/ijms20020390
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author Zupanska, Agata K.
LeFrois, Collin
Ferl, Robert J.
Paul, Anna-Lisa
author_facet Zupanska, Agata K.
LeFrois, Collin
Ferl, Robert J.
Paul, Anna-Lisa
author_sort Zupanska, Agata K.
collection PubMed
description Heat Shock Factor A2 (HsfA2) is part of the Heat Shock Factor (HSF) network, and plays an essential role beyond heat shock in environmental stress responses and cellular homeostatic control. Arabidopsis thaliana cell cultures derived from wild type (WT) ecotype Col-0 and a knockout line deficient in the gene encoding HSFA2 (HSFA2 KO) were grown aboard the International Space Station (ISS) to ascertain whether the HSF network functions in the adaptation to the novel environment of spaceflight. Microarray gene expression data were analyzed using a two-part comparative approach. First, genes differentially expressed between the two environments (spaceflight to ground) were identified within the same genotype, which represented physiological adaptation to spaceflight. Second, gene expression profiles were compared between the two genotypes (HSFA2 KO to WT) within the same environment, which defined genes uniquely required by each genotype on the ground and in spaceflight-adapted states. Results showed that the endoplasmic reticulum (ER) stress and unfolded protein response (UPR) define the HSFA2 KO cells’ physiological state irrespective of the environment, and likely resulted from a deficiency in the chaperone-mediated protein folding machinery in the mutant. Results further suggested that additional to its universal stress response role, HsfA2 also has specific roles in the physiological adaptation to spaceflight through cell wall remodeling, signal perception and transduction, and starch biosynthesis. Disabling HsfA2 altered the physiological state of the cells, and impacted the mechanisms induced to adapt to spaceflight, and identified HsfA2-dependent genes that are important to the adaption of wild type cells to spaceflight. Collectively these data indicate a non-thermal role for the HSF network in spaceflight adaptation.
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spelling pubmed-63590152019-02-06 HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight Zupanska, Agata K. LeFrois, Collin Ferl, Robert J. Paul, Anna-Lisa Int J Mol Sci Article Heat Shock Factor A2 (HsfA2) is part of the Heat Shock Factor (HSF) network, and plays an essential role beyond heat shock in environmental stress responses and cellular homeostatic control. Arabidopsis thaliana cell cultures derived from wild type (WT) ecotype Col-0 and a knockout line deficient in the gene encoding HSFA2 (HSFA2 KO) were grown aboard the International Space Station (ISS) to ascertain whether the HSF network functions in the adaptation to the novel environment of spaceflight. Microarray gene expression data were analyzed using a two-part comparative approach. First, genes differentially expressed between the two environments (spaceflight to ground) were identified within the same genotype, which represented physiological adaptation to spaceflight. Second, gene expression profiles were compared between the two genotypes (HSFA2 KO to WT) within the same environment, which defined genes uniquely required by each genotype on the ground and in spaceflight-adapted states. Results showed that the endoplasmic reticulum (ER) stress and unfolded protein response (UPR) define the HSFA2 KO cells’ physiological state irrespective of the environment, and likely resulted from a deficiency in the chaperone-mediated protein folding machinery in the mutant. Results further suggested that additional to its universal stress response role, HsfA2 also has specific roles in the physiological adaptation to spaceflight through cell wall remodeling, signal perception and transduction, and starch biosynthesis. Disabling HsfA2 altered the physiological state of the cells, and impacted the mechanisms induced to adapt to spaceflight, and identified HsfA2-dependent genes that are important to the adaption of wild type cells to spaceflight. Collectively these data indicate a non-thermal role for the HSF network in spaceflight adaptation. MDPI 2019-01-17 /pmc/articles/PMC6359015/ /pubmed/30658467 http://dx.doi.org/10.3390/ijms20020390 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zupanska, Agata K.
LeFrois, Collin
Ferl, Robert J.
Paul, Anna-Lisa
HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight
title HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight
title_full HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight
title_fullStr HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight
title_full_unstemmed HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight
title_short HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight
title_sort hsfa2 functions in the physiological adaptation of undifferentiated plant cells to spaceflight
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359015/
https://www.ncbi.nlm.nih.gov/pubmed/30658467
http://dx.doi.org/10.3390/ijms20020390
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