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Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery

Phytohormone abscisic acid (ABA) is the canonical trigger for stomatal closure upon abiotic stresses like drought. Soil-drying is known to facilitate root-to-shoot transport of sulfate. Remarkably, sulfate and sulfide—a downstream product of sulfate assimilation—have been independently shown to prom...

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Autores principales: Rajab, Hala, Khan, Muhammad Sayyar, Malagoli, Mario, Hell, Rüdiger, Wirtz, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359059/
https://www.ncbi.nlm.nih.gov/pubmed/30654485
http://dx.doi.org/10.3390/plants8010021
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author Rajab, Hala
Khan, Muhammad Sayyar
Malagoli, Mario
Hell, Rüdiger
Wirtz, Markus
author_facet Rajab, Hala
Khan, Muhammad Sayyar
Malagoli, Mario
Hell, Rüdiger
Wirtz, Markus
author_sort Rajab, Hala
collection PubMed
description Phytohormone abscisic acid (ABA) is the canonical trigger for stomatal closure upon abiotic stresses like drought. Soil-drying is known to facilitate root-to-shoot transport of sulfate. Remarkably, sulfate and sulfide—a downstream product of sulfate assimilation—have been independently shown to promote stomatal closure. For induction of stomatal closure, sulfate must be incorporated into cysteine, which triggers ABA biosynthesis by transcriptional activation of NCED3. Here, we apply reverse genetics to unravel if the canonical ABA signal transduction machinery is required for sulfate-induced stomata closure, and if cysteine biosynthesis is also mandatory for the induction of stomatal closure by the gasotransmitter sulfide. We provide genetic evidence for the importance of reactive oxygen species (ROS) production by the plasma membrane-localized NADPH oxidases, RBOHD, and RBOHF, during the sulfate-induced stomatal closure. In agreement with the established role of ROS as the second messenger of ABA-signaling, the SnRK2-type kinase OST1 and the protein phosphatase ABI1 are essential for sulfate-induced stomata closure. Finally, we show that sulfide fails to close stomata in a cysteine-biosynthesis depleted mutant. Our data support the hypothesis that the two mobile signals, sulfate and sulfide, induce stomatal closure by stimulating cysteine synthesis to trigger ABA production.
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spelling pubmed-63590592019-02-11 Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery Rajab, Hala Khan, Muhammad Sayyar Malagoli, Mario Hell, Rüdiger Wirtz, Markus Plants (Basel) Article Phytohormone abscisic acid (ABA) is the canonical trigger for stomatal closure upon abiotic stresses like drought. Soil-drying is known to facilitate root-to-shoot transport of sulfate. Remarkably, sulfate and sulfide—a downstream product of sulfate assimilation—have been independently shown to promote stomatal closure. For induction of stomatal closure, sulfate must be incorporated into cysteine, which triggers ABA biosynthesis by transcriptional activation of NCED3. Here, we apply reverse genetics to unravel if the canonical ABA signal transduction machinery is required for sulfate-induced stomata closure, and if cysteine biosynthesis is also mandatory for the induction of stomatal closure by the gasotransmitter sulfide. We provide genetic evidence for the importance of reactive oxygen species (ROS) production by the plasma membrane-localized NADPH oxidases, RBOHD, and RBOHF, during the sulfate-induced stomatal closure. In agreement with the established role of ROS as the second messenger of ABA-signaling, the SnRK2-type kinase OST1 and the protein phosphatase ABI1 are essential for sulfate-induced stomata closure. Finally, we show that sulfide fails to close stomata in a cysteine-biosynthesis depleted mutant. Our data support the hypothesis that the two mobile signals, sulfate and sulfide, induce stomatal closure by stimulating cysteine synthesis to trigger ABA production. MDPI 2019-01-16 /pmc/articles/PMC6359059/ /pubmed/30654485 http://dx.doi.org/10.3390/plants8010021 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rajab, Hala
Khan, Muhammad Sayyar
Malagoli, Mario
Hell, Rüdiger
Wirtz, Markus
Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery
title Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery
title_full Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery
title_fullStr Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery
title_full_unstemmed Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery
title_short Sulfate-Induced Stomata Closure Requires the Canonical ABA Signal Transduction Machinery
title_sort sulfate-induced stomata closure requires the canonical aba signal transduction machinery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359059/
https://www.ncbi.nlm.nih.gov/pubmed/30654485
http://dx.doi.org/10.3390/plants8010021
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AT malagolimario sulfateinducedstomataclosurerequiresthecanonicalabasignaltransductionmachinery
AT hellrudiger sulfateinducedstomataclosurerequiresthecanonicalabasignaltransductionmachinery
AT wirtzmarkus sulfateinducedstomataclosurerequiresthecanonicalabasignaltransductionmachinery