Cargando…
Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs
Sepsis, the clinical manifestation of serious infection, may disturb normal brain development, especially in preterm infants with an immature brain. We hypothesized that neonatal sepsis induces systemic metabolic alterations that rapidly affect metabolic signatures in immature brain and cerebrospina...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359096/ https://www.ncbi.nlm.nih.gov/pubmed/30641988 http://dx.doi.org/10.3390/metabo9010013 |
_version_ | 1783392150624403456 |
---|---|
author | Alinaghi, Masoumeh Jiang, Ping-Ping Brunse, Anders Sangild, Per Torp Bertram, Hanne Christine |
author_facet | Alinaghi, Masoumeh Jiang, Ping-Ping Brunse, Anders Sangild, Per Torp Bertram, Hanne Christine |
author_sort | Alinaghi, Masoumeh |
collection | PubMed |
description | Sepsis, the clinical manifestation of serious infection, may disturb normal brain development, especially in preterm infants with an immature brain. We hypothesized that neonatal sepsis induces systemic metabolic alterations that rapidly affect metabolic signatures in immature brain and cerebrospinal fluid (CSF). Cesarean-delivered preterm pigs systemically received 10(9) CFU/kg Staphylococcus epidermidis (SE) and were provided total parenteral nutrition (n = 9) or enteral supplementation with bovine colostrum (n = 10) and compared with uninfected pigs receiving parenteral nutrition (n = 7). Plasma, CSF, and brain tissue samples were collected after 24 h and analyzed by (1)H NMR-based metabolomics. Both plasma and CSF metabolomes revealed SE-induced changes in metabolite levels that reflected a modified energy metabolism. Hence, increased plasma lactate, alanine, and succinate levels, as well as CSF lactate levels, were observed during SE infection (all p < 0.05, ANOVA analysis). Myo-inositol, a glucose derivative known for beneficial effects on lung maturation in preterm infants, was also increased in plasma and CSF following SE infection. Enteral colostrum supplementation attenuated the lactate accumulation in blood and CSF. Bloodstream infection in preterm newborns was found to induce a rapid metabolic shift in both plasma and CSF, which was modulated by colostrum feeding. |
format | Online Article Text |
id | pubmed-6359096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63590962019-02-11 Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs Alinaghi, Masoumeh Jiang, Ping-Ping Brunse, Anders Sangild, Per Torp Bertram, Hanne Christine Metabolites Article Sepsis, the clinical manifestation of serious infection, may disturb normal brain development, especially in preterm infants with an immature brain. We hypothesized that neonatal sepsis induces systemic metabolic alterations that rapidly affect metabolic signatures in immature brain and cerebrospinal fluid (CSF). Cesarean-delivered preterm pigs systemically received 10(9) CFU/kg Staphylococcus epidermidis (SE) and were provided total parenteral nutrition (n = 9) or enteral supplementation with bovine colostrum (n = 10) and compared with uninfected pigs receiving parenteral nutrition (n = 7). Plasma, CSF, and brain tissue samples were collected after 24 h and analyzed by (1)H NMR-based metabolomics. Both plasma and CSF metabolomes revealed SE-induced changes in metabolite levels that reflected a modified energy metabolism. Hence, increased plasma lactate, alanine, and succinate levels, as well as CSF lactate levels, were observed during SE infection (all p < 0.05, ANOVA analysis). Myo-inositol, a glucose derivative known for beneficial effects on lung maturation in preterm infants, was also increased in plasma and CSF following SE infection. Enteral colostrum supplementation attenuated the lactate accumulation in blood and CSF. Bloodstream infection in preterm newborns was found to induce a rapid metabolic shift in both plasma and CSF, which was modulated by colostrum feeding. MDPI 2019-01-11 /pmc/articles/PMC6359096/ /pubmed/30641988 http://dx.doi.org/10.3390/metabo9010013 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alinaghi, Masoumeh Jiang, Ping-Ping Brunse, Anders Sangild, Per Torp Bertram, Hanne Christine Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs |
title | Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs |
title_full | Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs |
title_fullStr | Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs |
title_full_unstemmed | Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs |
title_short | Rapid Cerebral Metabolic Shift during Neonatal Sepsis Is Attenuated by Enteral Colostrum Supplementation in Preterm Pigs |
title_sort | rapid cerebral metabolic shift during neonatal sepsis is attenuated by enteral colostrum supplementation in preterm pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359096/ https://www.ncbi.nlm.nih.gov/pubmed/30641988 http://dx.doi.org/10.3390/metabo9010013 |
work_keys_str_mv | AT alinaghimasoumeh rapidcerebralmetabolicshiftduringneonatalsepsisisattenuatedbyenteralcolostrumsupplementationinpretermpigs AT jiangpingping rapidcerebralmetabolicshiftduringneonatalsepsisisattenuatedbyenteralcolostrumsupplementationinpretermpigs AT brunseanders rapidcerebralmetabolicshiftduringneonatalsepsisisattenuatedbyenteralcolostrumsupplementationinpretermpigs AT sangildpertorp rapidcerebralmetabolicshiftduringneonatalsepsisisattenuatedbyenteralcolostrumsupplementationinpretermpigs AT bertramhannechristine rapidcerebralmetabolicshiftduringneonatalsepsisisattenuatedbyenteralcolostrumsupplementationinpretermpigs |