Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture

Endogenous γ-aminobutyric acid (GABA)-dependent activity induces death of developing Purkinje neurons in mouse organotypic cerebellar cultures and the synthetic steroid mifepristone blocks this effect. Here, using brain-derived neurotrophic factor (BDNF) heterozygous mice, we show that BDNF plays no...

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Autores principales: Rakotomamonjy, Jennifer, Ghoumari, Abdel Mouman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359295/
https://www.ncbi.nlm.nih.gov/pubmed/30642045
http://dx.doi.org/10.3390/ijms20020285
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author Rakotomamonjy, Jennifer
Ghoumari, Abdel Mouman
author_facet Rakotomamonjy, Jennifer
Ghoumari, Abdel Mouman
author_sort Rakotomamonjy, Jennifer
collection PubMed
description Endogenous γ-aminobutyric acid (GABA)-dependent activity induces death of developing Purkinje neurons in mouse organotypic cerebellar cultures and the synthetic steroid mifepristone blocks this effect. Here, using brain-derived neurotrophic factor (BDNF) heterozygous mice, we show that BDNF plays no role in immature Purkinje cell death. However, interestingly, BDNF haploinsufficiency impairs neuronal survival induced by mifepristone and GABA(A)-receptors antagonist (bicuculline) treatments, indicating that the underlying neuroprotective mechanism requires the neurotrophin full expression.
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spelling pubmed-63592952019-02-06 Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture Rakotomamonjy, Jennifer Ghoumari, Abdel Mouman Int J Mol Sci Communication Endogenous γ-aminobutyric acid (GABA)-dependent activity induces death of developing Purkinje neurons in mouse organotypic cerebellar cultures and the synthetic steroid mifepristone blocks this effect. Here, using brain-derived neurotrophic factor (BDNF) heterozygous mice, we show that BDNF plays no role in immature Purkinje cell death. However, interestingly, BDNF haploinsufficiency impairs neuronal survival induced by mifepristone and GABA(A)-receptors antagonist (bicuculline) treatments, indicating that the underlying neuroprotective mechanism requires the neurotrophin full expression. MDPI 2019-01-12 /pmc/articles/PMC6359295/ /pubmed/30642045 http://dx.doi.org/10.3390/ijms20020285 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Rakotomamonjy, Jennifer
Ghoumari, Abdel Mouman
Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture
title Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture
title_full Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture
title_fullStr Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture
title_full_unstemmed Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture
title_short Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture
title_sort brain-derived neurotrophic factor is required for the neuroprotective effect of mifepristone on immature purkinje cells in cerebellar slice culture
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359295/
https://www.ncbi.nlm.nih.gov/pubmed/30642045
http://dx.doi.org/10.3390/ijms20020285
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AT ghoumariabdelmouman brainderivedneurotrophicfactorisrequiredfortheneuroprotectiveeffectofmifepristoneonimmaturepurkinjecellsincerebellarsliceculture

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