Cargando…
Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells
(1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease partic...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359328/ https://www.ncbi.nlm.nih.gov/pubmed/30658511 http://dx.doi.org/10.3390/molecules24020332 |
_version_ | 1783392217407160320 |
---|---|
author | Rahmani, Saher Budimir, Jelena Sejalon, Mylene Daurat, Morgane Aggad, Dina Vives, Eric Raehm, Laurence Garcia, Marcel Lichon, Laure Gary-Bobo, Magali Durand, Jean-Olivier Charnay, Clarence |
author_facet | Rahmani, Saher Budimir, Jelena Sejalon, Mylene Daurat, Morgane Aggad, Dina Vives, Eric Raehm, Laurence Garcia, Marcel Lichon, Laure Gary-Bobo, Magali Durand, Jean-Olivier Charnay, Clarence |
author_sort | Rahmani, Saher |
collection | PubMed |
description | (1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides. |
format | Online Article Text |
id | pubmed-6359328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63593282019-02-06 Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells Rahmani, Saher Budimir, Jelena Sejalon, Mylene Daurat, Morgane Aggad, Dina Vives, Eric Raehm, Laurence Garcia, Marcel Lichon, Laure Gary-Bobo, Magali Durand, Jean-Olivier Charnay, Clarence Molecules Article (1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides. MDPI 2019-01-17 /pmc/articles/PMC6359328/ /pubmed/30658511 http://dx.doi.org/10.3390/molecules24020332 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rahmani, Saher Budimir, Jelena Sejalon, Mylene Daurat, Morgane Aggad, Dina Vives, Eric Raehm, Laurence Garcia, Marcel Lichon, Laure Gary-Bobo, Magali Durand, Jean-Olivier Charnay, Clarence Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells |
title | Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells |
title_full | Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells |
title_fullStr | Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells |
title_full_unstemmed | Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells |
title_short | Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells |
title_sort | large pore mesoporous silica and organosilica nanoparticles for pepstatin a delivery in breast cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359328/ https://www.ncbi.nlm.nih.gov/pubmed/30658511 http://dx.doi.org/10.3390/molecules24020332 |
work_keys_str_mv | AT rahmanisaher largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT budimirjelena largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT sejalonmylene largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT dauratmorgane largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT aggaddina largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT viveseric largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT raehmlaurence largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT garciamarcel largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT lichonlaure largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT garybobomagali largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT durandjeanolivier largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells AT charnayclarence largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells |