Cargando…

Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

(1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease partic...

Descripción completa

Detalles Bibliográficos
Autores principales: Rahmani, Saher, Budimir, Jelena, Sejalon, Mylene, Daurat, Morgane, Aggad, Dina, Vives, Eric, Raehm, Laurence, Garcia, Marcel, Lichon, Laure, Gary-Bobo, Magali, Durand, Jean-Olivier, Charnay, Clarence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359328/
https://www.ncbi.nlm.nih.gov/pubmed/30658511
http://dx.doi.org/10.3390/molecules24020332
_version_ 1783392217407160320
author Rahmani, Saher
Budimir, Jelena
Sejalon, Mylene
Daurat, Morgane
Aggad, Dina
Vives, Eric
Raehm, Laurence
Garcia, Marcel
Lichon, Laure
Gary-Bobo, Magali
Durand, Jean-Olivier
Charnay, Clarence
author_facet Rahmani, Saher
Budimir, Jelena
Sejalon, Mylene
Daurat, Morgane
Aggad, Dina
Vives, Eric
Raehm, Laurence
Garcia, Marcel
Lichon, Laure
Gary-Bobo, Magali
Durand, Jean-Olivier
Charnay, Clarence
author_sort Rahmani, Saher
collection PubMed
description (1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides.
format Online
Article
Text
id pubmed-6359328
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63593282019-02-06 Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells Rahmani, Saher Budimir, Jelena Sejalon, Mylene Daurat, Morgane Aggad, Dina Vives, Eric Raehm, Laurence Garcia, Marcel Lichon, Laure Gary-Bobo, Magali Durand, Jean-Olivier Charnay, Clarence Molecules Article (1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides. MDPI 2019-01-17 /pmc/articles/PMC6359328/ /pubmed/30658511 http://dx.doi.org/10.3390/molecules24020332 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rahmani, Saher
Budimir, Jelena
Sejalon, Mylene
Daurat, Morgane
Aggad, Dina
Vives, Eric
Raehm, Laurence
Garcia, Marcel
Lichon, Laure
Gary-Bobo, Magali
Durand, Jean-Olivier
Charnay, Clarence
Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells
title Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells
title_full Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells
title_fullStr Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells
title_full_unstemmed Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells
title_short Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells
title_sort large pore mesoporous silica and organosilica nanoparticles for pepstatin a delivery in breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359328/
https://www.ncbi.nlm.nih.gov/pubmed/30658511
http://dx.doi.org/10.3390/molecules24020332
work_keys_str_mv AT rahmanisaher largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT budimirjelena largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT sejalonmylene largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT dauratmorgane largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT aggaddina largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT viveseric largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT raehmlaurence largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT garciamarcel largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT lichonlaure largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT garybobomagali largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT durandjeanolivier largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells
AT charnayclarence largeporemesoporoussilicaandorganosilicananoparticlesforpepstatinadeliveryinbreastcancercells