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Expanding the Genetic Code for Site-Directed Spin-Labeling
Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy enables studies of the structure, dynamics, and interactions of proteins in the noncrystalline state. The scope and analytical value of SDSL–EPR experiments crucially depends on the employed lab...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359334/ https://www.ncbi.nlm.nih.gov/pubmed/30654584 http://dx.doi.org/10.3390/ijms20020373 |
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author | Braun, Theresa Drescher, Malte Summerer, Daniel |
author_facet | Braun, Theresa Drescher, Malte Summerer, Daniel |
author_sort | Braun, Theresa |
collection | PubMed |
description | Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy enables studies of the structure, dynamics, and interactions of proteins in the noncrystalline state. The scope and analytical value of SDSL–EPR experiments crucially depends on the employed labeling strategy, with key aspects being labeling chemoselectivity and biocompatibility, as well as stability and spectroscopic properties of the resulting label. The use of genetically encoded noncanonical amino acids (ncAA) is an emerging strategy for SDSL that holds great promise for providing excellent chemoselectivity and potential for experiments in complex biological environments such as living cells. We here give a focused overview of recent advancements in this field and discuss their potentials and challenges for advancing SDSL–EPR studies. |
format | Online Article Text |
id | pubmed-6359334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63593342019-02-06 Expanding the Genetic Code for Site-Directed Spin-Labeling Braun, Theresa Drescher, Malte Summerer, Daniel Int J Mol Sci Review Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy enables studies of the structure, dynamics, and interactions of proteins in the noncrystalline state. The scope and analytical value of SDSL–EPR experiments crucially depends on the employed labeling strategy, with key aspects being labeling chemoselectivity and biocompatibility, as well as stability and spectroscopic properties of the resulting label. The use of genetically encoded noncanonical amino acids (ncAA) is an emerging strategy for SDSL that holds great promise for providing excellent chemoselectivity and potential for experiments in complex biological environments such as living cells. We here give a focused overview of recent advancements in this field and discuss their potentials and challenges for advancing SDSL–EPR studies. MDPI 2019-01-16 /pmc/articles/PMC6359334/ /pubmed/30654584 http://dx.doi.org/10.3390/ijms20020373 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Braun, Theresa Drescher, Malte Summerer, Daniel Expanding the Genetic Code for Site-Directed Spin-Labeling |
title | Expanding the Genetic Code for Site-Directed Spin-Labeling |
title_full | Expanding the Genetic Code for Site-Directed Spin-Labeling |
title_fullStr | Expanding the Genetic Code for Site-Directed Spin-Labeling |
title_full_unstemmed | Expanding the Genetic Code for Site-Directed Spin-Labeling |
title_short | Expanding the Genetic Code for Site-Directed Spin-Labeling |
title_sort | expanding the genetic code for site-directed spin-labeling |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359334/ https://www.ncbi.nlm.nih.gov/pubmed/30654584 http://dx.doi.org/10.3390/ijms20020373 |
work_keys_str_mv | AT brauntheresa expandingthegeneticcodeforsitedirectedspinlabeling AT dreschermalte expandingthegeneticcodeforsitedirectedspinlabeling AT summererdaniel expandingthegeneticcodeforsitedirectedspinlabeling |