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Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration
Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359357/ https://www.ncbi.nlm.nih.gov/pubmed/30597924 http://dx.doi.org/10.3390/biom9010011 |
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author | Klett, Hagen Jürgensen, Lonny Most, Patrick Busch, Martin Günther, Fabian Dobreva, Gergana Leuschner, Florian Hassel, David Busch, Hauke Boerries, Melanie |
author_facet | Klett, Hagen Jürgensen, Lonny Most, Patrick Busch, Martin Günther, Fabian Dobreva, Gergana Leuschner, Florian Hassel, David Busch, Hauke Boerries, Melanie |
author_sort | Klett, Hagen |
collection | PubMed |
description | Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1–160 days post cryoinjury (dpi). Using a control model of sham-operated and healthy fish, we extracted the regeneration specific response and further delineated the spatio-temporal organization of regeneration processes such as cell cycle and heart function. In addition, we identified novel (miR-148/152, miR-218b and miR-19) and previously known microRNAs among the top regulators of heart regeneration by using theoretically predicted target sites and correlation of expression profiles from both mRNA and microRNA. In a cross-species effort, we validated our findings in the dynamic process of rat myoblasts differentiating into cardiomyocytes-like cells (H9c2 cell line). Concluding, we elucidated different phases of transcriptomic responses during zebrafish heart regeneration. Furthermore, microRNAs showed to be important regulators in cardiomyocyte proliferation over time. |
format | Online Article Text |
id | pubmed-6359357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63593572019-02-11 Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration Klett, Hagen Jürgensen, Lonny Most, Patrick Busch, Martin Günther, Fabian Dobreva, Gergana Leuschner, Florian Hassel, David Busch, Hauke Boerries, Melanie Biomolecules Article Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1–160 days post cryoinjury (dpi). Using a control model of sham-operated and healthy fish, we extracted the regeneration specific response and further delineated the spatio-temporal organization of regeneration processes such as cell cycle and heart function. In addition, we identified novel (miR-148/152, miR-218b and miR-19) and previously known microRNAs among the top regulators of heart regeneration by using theoretically predicted target sites and correlation of expression profiles from both mRNA and microRNA. In a cross-species effort, we validated our findings in the dynamic process of rat myoblasts differentiating into cardiomyocytes-like cells (H9c2 cell line). Concluding, we elucidated different phases of transcriptomic responses during zebrafish heart regeneration. Furthermore, microRNAs showed to be important regulators in cardiomyocyte proliferation over time. MDPI 2018-12-28 /pmc/articles/PMC6359357/ /pubmed/30597924 http://dx.doi.org/10.3390/biom9010011 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Klett, Hagen Jürgensen, Lonny Most, Patrick Busch, Martin Günther, Fabian Dobreva, Gergana Leuschner, Florian Hassel, David Busch, Hauke Boerries, Melanie Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration |
title | Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration |
title_full | Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration |
title_fullStr | Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration |
title_full_unstemmed | Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration |
title_short | Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration |
title_sort | delineating the dynamic transcriptome response of mrna and microrna during zebrafish heart regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359357/ https://www.ncbi.nlm.nih.gov/pubmed/30597924 http://dx.doi.org/10.3390/biom9010011 |
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