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Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration

Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating the...

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Autores principales: Klett, Hagen, Jürgensen, Lonny, Most, Patrick, Busch, Martin, Günther, Fabian, Dobreva, Gergana, Leuschner, Florian, Hassel, David, Busch, Hauke, Boerries, Melanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359357/
https://www.ncbi.nlm.nih.gov/pubmed/30597924
http://dx.doi.org/10.3390/biom9010011
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author Klett, Hagen
Jürgensen, Lonny
Most, Patrick
Busch, Martin
Günther, Fabian
Dobreva, Gergana
Leuschner, Florian
Hassel, David
Busch, Hauke
Boerries, Melanie
author_facet Klett, Hagen
Jürgensen, Lonny
Most, Patrick
Busch, Martin
Günther, Fabian
Dobreva, Gergana
Leuschner, Florian
Hassel, David
Busch, Hauke
Boerries, Melanie
author_sort Klett, Hagen
collection PubMed
description Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1–160 days post cryoinjury (dpi). Using a control model of sham-operated and healthy fish, we extracted the regeneration specific response and further delineated the spatio-temporal organization of regeneration processes such as cell cycle and heart function. In addition, we identified novel (miR-148/152, miR-218b and miR-19) and previously known microRNAs among the top regulators of heart regeneration by using theoretically predicted target sites and correlation of expression profiles from both mRNA and microRNA. In a cross-species effort, we validated our findings in the dynamic process of rat myoblasts differentiating into cardiomyocytes-like cells (H9c2 cell line). Concluding, we elucidated different phases of transcriptomic responses during zebrafish heart regeneration. Furthermore, microRNAs showed to be important regulators in cardiomyocyte proliferation over time.
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spelling pubmed-63593572019-02-11 Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration Klett, Hagen Jürgensen, Lonny Most, Patrick Busch, Martin Günther, Fabian Dobreva, Gergana Leuschner, Florian Hassel, David Busch, Hauke Boerries, Melanie Biomolecules Article Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1–160 days post cryoinjury (dpi). Using a control model of sham-operated and healthy fish, we extracted the regeneration specific response and further delineated the spatio-temporal organization of regeneration processes such as cell cycle and heart function. In addition, we identified novel (miR-148/152, miR-218b and miR-19) and previously known microRNAs among the top regulators of heart regeneration by using theoretically predicted target sites and correlation of expression profiles from both mRNA and microRNA. In a cross-species effort, we validated our findings in the dynamic process of rat myoblasts differentiating into cardiomyocytes-like cells (H9c2 cell line). Concluding, we elucidated different phases of transcriptomic responses during zebrafish heart regeneration. Furthermore, microRNAs showed to be important regulators in cardiomyocyte proliferation over time. MDPI 2018-12-28 /pmc/articles/PMC6359357/ /pubmed/30597924 http://dx.doi.org/10.3390/biom9010011 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Klett, Hagen
Jürgensen, Lonny
Most, Patrick
Busch, Martin
Günther, Fabian
Dobreva, Gergana
Leuschner, Florian
Hassel, David
Busch, Hauke
Boerries, Melanie
Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration
title Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration
title_full Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration
title_fullStr Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration
title_full_unstemmed Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration
title_short Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration
title_sort delineating the dynamic transcriptome response of mrna and microrna during zebrafish heart regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359357/
https://www.ncbi.nlm.nih.gov/pubmed/30597924
http://dx.doi.org/10.3390/biom9010011
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