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The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species

Magnolia zenii is a critically endangered species known from only 18 trees that survive on Baohua Mountain in Jiangsu province, China. Little information is available regarding its molecular biology, with no genomic study performed on M. zenii until now. We determined the complete plastid genome of...

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Autores principales: Li, Yongfu, Sylvester, Steven Paul, Li, Meng, Zhang, Cheng, Li, Xuan, Duan, Yifan, Wang, Xianrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359370/
https://www.ncbi.nlm.nih.gov/pubmed/30641990
http://dx.doi.org/10.3390/molecules24020261
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author Li, Yongfu
Sylvester, Steven Paul
Li, Meng
Zhang, Cheng
Li, Xuan
Duan, Yifan
Wang, Xianrong
author_facet Li, Yongfu
Sylvester, Steven Paul
Li, Meng
Zhang, Cheng
Li, Xuan
Duan, Yifan
Wang, Xianrong
author_sort Li, Yongfu
collection PubMed
description Magnolia zenii is a critically endangered species known from only 18 trees that survive on Baohua Mountain in Jiangsu province, China. Little information is available regarding its molecular biology, with no genomic study performed on M. zenii until now. We determined the complete plastid genome of M. zenii and identified microsatellites. Whole sequence alignment and phylogenetic analysis using BI and ML methods were also conducted. The plastome of M. zenii was 160,048 bp long with 39.2% GC content and included a pair of inverted repeats (IRs) of 26,596 bp that separated a large single-copy (LSC) region of 88,098 bp and a small single-copy (SSC) region of 18,757 bp. One hundred thirty genes were identified, of which 79 were protein-coding genes, 37 were transfer RNAs, and eight were ribosomal RNAs. Thirty seven simple sequence repeats (SSRs) were also identified. Comparative analyses of genome structure and sequence data of closely-related species revealed five mutation hotspots, useful for future phylogenetic research. Magnolia zenii was placed as sister to M. biondii with strong support in all analyses. Overall, this study providing M. zenii genomic resources will be beneficial for the evolutionary study and phylogenetic reconstruction of Magnoliaceae.
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spelling pubmed-63593702019-02-06 The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species Li, Yongfu Sylvester, Steven Paul Li, Meng Zhang, Cheng Li, Xuan Duan, Yifan Wang, Xianrong Molecules Article Magnolia zenii is a critically endangered species known from only 18 trees that survive on Baohua Mountain in Jiangsu province, China. Little information is available regarding its molecular biology, with no genomic study performed on M. zenii until now. We determined the complete plastid genome of M. zenii and identified microsatellites. Whole sequence alignment and phylogenetic analysis using BI and ML methods were also conducted. The plastome of M. zenii was 160,048 bp long with 39.2% GC content and included a pair of inverted repeats (IRs) of 26,596 bp that separated a large single-copy (LSC) region of 88,098 bp and a small single-copy (SSC) region of 18,757 bp. One hundred thirty genes were identified, of which 79 were protein-coding genes, 37 were transfer RNAs, and eight were ribosomal RNAs. Thirty seven simple sequence repeats (SSRs) were also identified. Comparative analyses of genome structure and sequence data of closely-related species revealed five mutation hotspots, useful for future phylogenetic research. Magnolia zenii was placed as sister to M. biondii with strong support in all analyses. Overall, this study providing M. zenii genomic resources will be beneficial for the evolutionary study and phylogenetic reconstruction of Magnoliaceae. MDPI 2019-01-11 /pmc/articles/PMC6359370/ /pubmed/30641990 http://dx.doi.org/10.3390/molecules24020261 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yongfu
Sylvester, Steven Paul
Li, Meng
Zhang, Cheng
Li, Xuan
Duan, Yifan
Wang, Xianrong
The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species
title The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species
title_full The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species
title_fullStr The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species
title_full_unstemmed The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species
title_short The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species
title_sort complete plastid genome of magnolia zenii and genetic comparison to magnoliaceae species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359370/
https://www.ncbi.nlm.nih.gov/pubmed/30641990
http://dx.doi.org/10.3390/molecules24020261
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