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Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12
Magnesium (Mg)-based biomaterials are promising candidates for bone and tissue regeneration. Alloying and surface modifications provide effective strategies for optimizing and tailoring their degradation kinetics. Nevertheless, biocompatibility analyses of Mg-based materials are challenging due to i...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359522/ https://www.ncbi.nlm.nih.gov/pubmed/30634646 http://dx.doi.org/10.3390/ijms20020255 |
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author | Jung, Ole Smeets, Ralf Hartjen, Philip Schnettler, Reinhard Feyerabend, Frank Klein, Martin Wegner, Nils Walther, Frank Stangier, Dominic Henningsen, Anders Rendenbach, Carsten Heiland, Max Barbeck, Mike Kopp, Alexander |
author_facet | Jung, Ole Smeets, Ralf Hartjen, Philip Schnettler, Reinhard Feyerabend, Frank Klein, Martin Wegner, Nils Walther, Frank Stangier, Dominic Henningsen, Anders Rendenbach, Carsten Heiland, Max Barbeck, Mike Kopp, Alexander |
author_sort | Jung, Ole |
collection | PubMed |
description | Magnesium (Mg)-based biomaterials are promising candidates for bone and tissue regeneration. Alloying and surface modifications provide effective strategies for optimizing and tailoring their degradation kinetics. Nevertheless, biocompatibility analyses of Mg-based materials are challenging due to its special degradation mechanism with continuous hydrogen release. In this context, the hydrogen release and the related (micro-) milieu conditions pretend to strictly follow in vitro standards based on ISO 10993-5/-12. Thus, special adaptions for the testing of Mg materials are necessary, which have been described in a previous study from our group. Based on these adaptions, further developments of a test procedure allowing rapid and effective in vitro cytocompatibility analyses of Mg-based materials based on ISO 10993-5/-12 are necessary. The following study introduces a new two-step test scheme for rapid and effective testing of Mg. Specimens with different surface characteristics were produced by means of plasma electrolytic oxidation (PEO) using silicate-based and phosphate-based electrolytes. The test samples were evaluated for corrosion behavior, cytocompatibility and their mechanical and osteogenic properties. Thereby, two PEO ceramics could be identified for further in vivo evaluations. |
format | Online Article Text |
id | pubmed-6359522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63595222019-02-06 Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12 Jung, Ole Smeets, Ralf Hartjen, Philip Schnettler, Reinhard Feyerabend, Frank Klein, Martin Wegner, Nils Walther, Frank Stangier, Dominic Henningsen, Anders Rendenbach, Carsten Heiland, Max Barbeck, Mike Kopp, Alexander Int J Mol Sci Article Magnesium (Mg)-based biomaterials are promising candidates for bone and tissue regeneration. Alloying and surface modifications provide effective strategies for optimizing and tailoring their degradation kinetics. Nevertheless, biocompatibility analyses of Mg-based materials are challenging due to its special degradation mechanism with continuous hydrogen release. In this context, the hydrogen release and the related (micro-) milieu conditions pretend to strictly follow in vitro standards based on ISO 10993-5/-12. Thus, special adaptions for the testing of Mg materials are necessary, which have been described in a previous study from our group. Based on these adaptions, further developments of a test procedure allowing rapid and effective in vitro cytocompatibility analyses of Mg-based materials based on ISO 10993-5/-12 are necessary. The following study introduces a new two-step test scheme for rapid and effective testing of Mg. Specimens with different surface characteristics were produced by means of plasma electrolytic oxidation (PEO) using silicate-based and phosphate-based electrolytes. The test samples were evaluated for corrosion behavior, cytocompatibility and their mechanical and osteogenic properties. Thereby, two PEO ceramics could be identified for further in vivo evaluations. MDPI 2019-01-10 /pmc/articles/PMC6359522/ /pubmed/30634646 http://dx.doi.org/10.3390/ijms20020255 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jung, Ole Smeets, Ralf Hartjen, Philip Schnettler, Reinhard Feyerabend, Frank Klein, Martin Wegner, Nils Walther, Frank Stangier, Dominic Henningsen, Anders Rendenbach, Carsten Heiland, Max Barbeck, Mike Kopp, Alexander Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12 |
title | Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12 |
title_full | Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12 |
title_fullStr | Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12 |
title_full_unstemmed | Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12 |
title_short | Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12 |
title_sort | improved in vitro test procedure for full assessment of the cytocompatibility of degradable magnesium based on iso 10993-5/-12 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359522/ https://www.ncbi.nlm.nih.gov/pubmed/30634646 http://dx.doi.org/10.3390/ijms20020255 |
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