Cargando…
Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA
BACKGROUND: Baduanjin, a traditional Chinese exercise therapy, has been widely used in China to treat type 2 diabetes (T2DM) with depression (DD). However, the underlying mechanism of Baduanjin in anti-DD is unclear. This study was focused on investigating the effects of Baduanjin on symptoms of dep...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359771/ https://www.ncbi.nlm.nih.gov/pubmed/30733823 http://dx.doi.org/10.1186/s13020-019-0225-1 |
_version_ | 1783392343601184768 |
---|---|
author | An, Tian He, Zhong-Chen Zhang, Xin-Qing Li, Jun Chen, Ai-Ling Tan, Fang Chen, Hong-Dong Lv, Bo-Han Lian, Juan Gao, Si-Hua Jiang, Guang-Jian |
author_facet | An, Tian He, Zhong-Chen Zhang, Xin-Qing Li, Jun Chen, Ai-Ling Tan, Fang Chen, Hong-Dong Lv, Bo-Han Lian, Juan Gao, Si-Hua Jiang, Guang-Jian |
author_sort | An, Tian |
collection | PubMed |
description | BACKGROUND: Baduanjin, a traditional Chinese exercise therapy, has been widely used in China to treat type 2 diabetes (T2DM) with depression (DD). However, the underlying mechanism of Baduanjin in anti-DD is unclear. This study was focused on investigating the effects of Baduanjin on symptoms of depression and blood glucose in patients with DD and the underlying mechanism. METHODS: We performed a 12-week Baduanjin intervention on patients with DD and longitudinally compared the differential expressions of lncRNAs, circRNAs, and mRNAs between pre- (BDD) and post- (ADD) Baduanjin intervention in the same group. Subsequently, Gene Ontology (GO) and pathway analysis was performed to investigate the function of differentially expressed mRNAs. Finally, Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to verify the sequencing result and the mRNA-lncRNA regulatory network was constructed. RESULTS: The blood glucose level, depression index scores, and PHQ9 scores of the patients with DD were significantly decreased (P < 0.05) after Baduanjin intervention. Compared to BDD, 207 lncRNAs, 266 circRNAs, and 610 differentially expressed mRNAs were identified in ADD. Kyoto Encyclopedia of Genes and Genomes (KEGG) and GO showed that the significantly dysregulated mRNAs were mainly involved in immune function and inflammatory response pathways, and various signaling pathways including IL-17 and TNF. In addition, we selected five differentially expressed lncRNAs to construct an lncRNA-mRNA regulatory network, and found a total of 1045 mRNAs associated with them. CONCLUSIONS: Our research is the first systematic profiling of mRNA, lncRNA, and circRNA in patients of ADD and BDD, and provides valuable insights in the potential mechanism of Baduanjin in anti-DD. Further, it was confirmed that Baduanjin is a safe and effective intervention for patients with DD because it can effectively ameliorate the symptoms of depression and blood glucose levels in patients with DD by regulating the dysregulated expression of lncRNA, mRNA, and circRNA. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-019-0225-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6359771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63597712019-02-07 Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA An, Tian He, Zhong-Chen Zhang, Xin-Qing Li, Jun Chen, Ai-Ling Tan, Fang Chen, Hong-Dong Lv, Bo-Han Lian, Juan Gao, Si-Hua Jiang, Guang-Jian Chin Med Research BACKGROUND: Baduanjin, a traditional Chinese exercise therapy, has been widely used in China to treat type 2 diabetes (T2DM) with depression (DD). However, the underlying mechanism of Baduanjin in anti-DD is unclear. This study was focused on investigating the effects of Baduanjin on symptoms of depression and blood glucose in patients with DD and the underlying mechanism. METHODS: We performed a 12-week Baduanjin intervention on patients with DD and longitudinally compared the differential expressions of lncRNAs, circRNAs, and mRNAs between pre- (BDD) and post- (ADD) Baduanjin intervention in the same group. Subsequently, Gene Ontology (GO) and pathway analysis was performed to investigate the function of differentially expressed mRNAs. Finally, Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to verify the sequencing result and the mRNA-lncRNA regulatory network was constructed. RESULTS: The blood glucose level, depression index scores, and PHQ9 scores of the patients with DD were significantly decreased (P < 0.05) after Baduanjin intervention. Compared to BDD, 207 lncRNAs, 266 circRNAs, and 610 differentially expressed mRNAs were identified in ADD. Kyoto Encyclopedia of Genes and Genomes (KEGG) and GO showed that the significantly dysregulated mRNAs were mainly involved in immune function and inflammatory response pathways, and various signaling pathways including IL-17 and TNF. In addition, we selected five differentially expressed lncRNAs to construct an lncRNA-mRNA regulatory network, and found a total of 1045 mRNAs associated with them. CONCLUSIONS: Our research is the first systematic profiling of mRNA, lncRNA, and circRNA in patients of ADD and BDD, and provides valuable insights in the potential mechanism of Baduanjin in anti-DD. Further, it was confirmed that Baduanjin is a safe and effective intervention for patients with DD because it can effectively ameliorate the symptoms of depression and blood glucose levels in patients with DD by regulating the dysregulated expression of lncRNA, mRNA, and circRNA. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-019-0225-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-01 /pmc/articles/PMC6359771/ /pubmed/30733823 http://dx.doi.org/10.1186/s13020-019-0225-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research An, Tian He, Zhong-Chen Zhang, Xin-Qing Li, Jun Chen, Ai-Ling Tan, Fang Chen, Hong-Dong Lv, Bo-Han Lian, Juan Gao, Si-Hua Jiang, Guang-Jian Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA |
title | Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA |
title_full | Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA |
title_fullStr | Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA |
title_full_unstemmed | Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA |
title_short | Baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mRNA, lncRNA, and circRNA |
title_sort | baduanjin exerts anti-diabetic and anti-depression effects by regulating the expression of mrna, lncrna, and circrna |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359771/ https://www.ncbi.nlm.nih.gov/pubmed/30733823 http://dx.doi.org/10.1186/s13020-019-0225-1 |
work_keys_str_mv | AT antian baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT hezhongchen baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT zhangxinqing baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT lijun baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT chenailing baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT tanfang baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT chenhongdong baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT lvbohan baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT lianjuan baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT gaosihua baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna AT jiangguangjian baduanjinexertsantidiabeticandantidepressioneffectsbyregulatingtheexpressionofmrnalncrnaandcircrna |