A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro

BACKGROUND: Porcine circovirus type 2 (PCV2) is the causal agent of postweaning multisystemic wasting syndrome (PMWS), causing large economical losses of the global swine industry. Nitric oxide (NO), as an important signaling molecule, has antiviral activity on some viruses. To date, there is little...

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Detalles Bibliográficos
Autores principales: Xue, Tao, Li, Jizong, Liu, Chuanmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359798/
https://www.ncbi.nlm.nih.gov/pubmed/30709350
http://dx.doi.org/10.1186/s12917-019-1796-x
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author Xue, Tao
Li, Jizong
Liu, Chuanmin
author_facet Xue, Tao
Li, Jizong
Liu, Chuanmin
author_sort Xue, Tao
collection PubMed
description BACKGROUND: Porcine circovirus type 2 (PCV2) is the causal agent of postweaning multisystemic wasting syndrome (PMWS), causing large economical losses of the global swine industry. Nitric oxide (NO), as an important signaling molecule, has antiviral activity on some viruses. To date, there is little information on the role of NO during PCV2 infection. RESULTS: We used indirect fluorescence assay (IFA), TCID(50), real-time RT-qPCR and western blot assay to reveal the role of NO in restricting PCV2 replication. PCV2 replication was inhibited by a form of NO, NO(•), whereas PCV2 was not susceptible to another form of NO, NO(+). CONCLUSION: Our findings indicate that the form of NO(•) has a potential role in the fight against PCV2 infection.
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spelling pubmed-63597982019-02-07 A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro Xue, Tao Li, Jizong Liu, Chuanmin BMC Vet Res Research Article BACKGROUND: Porcine circovirus type 2 (PCV2) is the causal agent of postweaning multisystemic wasting syndrome (PMWS), causing large economical losses of the global swine industry. Nitric oxide (NO), as an important signaling molecule, has antiviral activity on some viruses. To date, there is little information on the role of NO during PCV2 infection. RESULTS: We used indirect fluorescence assay (IFA), TCID(50), real-time RT-qPCR and western blot assay to reveal the role of NO in restricting PCV2 replication. PCV2 replication was inhibited by a form of NO, NO(•), whereas PCV2 was not susceptible to another form of NO, NO(+). CONCLUSION: Our findings indicate that the form of NO(•) has a potential role in the fight against PCV2 infection. BioMed Central 2019-02-01 /pmc/articles/PMC6359798/ /pubmed/30709350 http://dx.doi.org/10.1186/s12917-019-1796-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Xue, Tao
Li, Jizong
Liu, Chuanmin
A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro
title A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro
title_full A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro
title_fullStr A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro
title_full_unstemmed A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro
title_short A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro
title_sort radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359798/
https://www.ncbi.nlm.nih.gov/pubmed/30709350
http://dx.doi.org/10.1186/s12917-019-1796-x
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