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Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon
BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359860/ https://www.ncbi.nlm.nih.gov/pubmed/30710998 http://dx.doi.org/10.1186/s12866-019-1401-2 |
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author | Nawfal Dagher, Tania Al-Bayssari, Charbel Chabou, Selma Antar, Nadine Diene, Seydina M. Azar, Eid Rolain, Jean-Marc |
author_facet | Nawfal Dagher, Tania Al-Bayssari, Charbel Chabou, Selma Antar, Nadine Diene, Seydina M. Azar, Eid Rolain, Jean-Marc |
author_sort | Nawfal Dagher, Tania |
collection | PubMed |
description | BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. METHODS: Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis. RESULTS: Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D carbapenemase bla(oxa-23) gene while one isolate carried bla(oxa-72) gene. MLST results revealed three sequence types, namely ST2, ST699, and ST627. Isolates having ST2 were the most prevalent clone (29/31, 93.5%). CONCLUSIONS: This study shows a nosocomial spread of multidrug-resistant A. baumannii ST2 having bla(OXA-23) gene in Saint-George in Lebanon. Monitoring and control measures need to be adopted to avoid the spread of A. baumannii to patients. |
format | Online Article Text |
id | pubmed-6359860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63598602019-02-07 Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon Nawfal Dagher, Tania Al-Bayssari, Charbel Chabou, Selma Antar, Nadine Diene, Seydina M. Azar, Eid Rolain, Jean-Marc BMC Microbiol Research Article BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. METHODS: Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis. RESULTS: Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D carbapenemase bla(oxa-23) gene while one isolate carried bla(oxa-72) gene. MLST results revealed three sequence types, namely ST2, ST699, and ST627. Isolates having ST2 were the most prevalent clone (29/31, 93.5%). CONCLUSIONS: This study shows a nosocomial spread of multidrug-resistant A. baumannii ST2 having bla(OXA-23) gene in Saint-George in Lebanon. Monitoring and control measures need to be adopted to avoid the spread of A. baumannii to patients. BioMed Central 2019-02-02 /pmc/articles/PMC6359860/ /pubmed/30710998 http://dx.doi.org/10.1186/s12866-019-1401-2 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Nawfal Dagher, Tania Al-Bayssari, Charbel Chabou, Selma Antar, Nadine Diene, Seydina M. Azar, Eid Rolain, Jean-Marc Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
title | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
title_full | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
title_fullStr | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
title_full_unstemmed | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
title_short | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
title_sort | investigation of multidrug-resistant st2 acinetobacter baumannii isolated from saint george hospital in lebanon |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359860/ https://www.ncbi.nlm.nih.gov/pubmed/30710998 http://dx.doi.org/10.1186/s12866-019-1401-2 |
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