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Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator

Mammalian hibernators store fat extensively in white adipose tissues (WATs) during pre-hibernation period (Pre-HIB) to prepare for hibernation. However, the molecular mechanisms underlying the pre-hibernation remodeling of WAT have not been fully elucidated. Syrian hamsters, a food-storing hibernato...

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Autores principales: Chayama, Yuichi, Ando, Lisa, Sato, Yuya, Shigenobu, Shuji, Anegawa, Daisuke, Fujimoto, Takayuki, Taii, Hiroki, Tamura, Yutaka, Miura, Masayuki, Yamaguchi, Yoshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360343/
https://www.ncbi.nlm.nih.gov/pubmed/30745884
http://dx.doi.org/10.3389/fphys.2018.01973
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author Chayama, Yuichi
Ando, Lisa
Sato, Yuya
Shigenobu, Shuji
Anegawa, Daisuke
Fujimoto, Takayuki
Taii, Hiroki
Tamura, Yutaka
Miura, Masayuki
Yamaguchi, Yoshifumi
author_facet Chayama, Yuichi
Ando, Lisa
Sato, Yuya
Shigenobu, Shuji
Anegawa, Daisuke
Fujimoto, Takayuki
Taii, Hiroki
Tamura, Yutaka
Miura, Masayuki
Yamaguchi, Yoshifumi
author_sort Chayama, Yuichi
collection PubMed
description Mammalian hibernators store fat extensively in white adipose tissues (WATs) during pre-hibernation period (Pre-HIB) to prepare for hibernation. However, the molecular mechanisms underlying the pre-hibernation remodeling of WAT have not been fully elucidated. Syrian hamsters, a food-storing hibernator, can hibernate when exposed to a winter-like short day photoperiod and cold ambient temperature (SD-Cold). Animals subjected to prolonged SD-Cold had smaller white adipocytes and beige-like cells within subcutaneous inguinal WAT (iWAT). Time-course analysis of gene expression with RNA-sequencing and quantitative PCR demonstrated that the mRNA expression of not only genes involved in lipid catabolism (lipolysis and beta-oxidation) but also lipid anabolism (lipogenesis and lipid desaturation) was simultaneously up-regulated prior to hibernation onset in the animals. The enhanced capacity of both lipid catabolism and lipid anabolism during hibernation period (HIB) is striking contrast to previous observations in fat-storing hibernators that only enhance catabolism during HIB. The mRNA expression of mTORC1 and PPAR signaling molecules increased, and pharmacological activation of PPARs indeed up-regulated lipid metabolism genes in iWAT explants from Syrian hamsters. These results suggest that the Syrian hamster rewires lipid metabolisms while preparing for hibernation to effectively utilize body fat and synthesize it from food intake during HIB.
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spelling pubmed-63603432019-02-11 Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator Chayama, Yuichi Ando, Lisa Sato, Yuya Shigenobu, Shuji Anegawa, Daisuke Fujimoto, Takayuki Taii, Hiroki Tamura, Yutaka Miura, Masayuki Yamaguchi, Yoshifumi Front Physiol Physiology Mammalian hibernators store fat extensively in white adipose tissues (WATs) during pre-hibernation period (Pre-HIB) to prepare for hibernation. However, the molecular mechanisms underlying the pre-hibernation remodeling of WAT have not been fully elucidated. Syrian hamsters, a food-storing hibernator, can hibernate when exposed to a winter-like short day photoperiod and cold ambient temperature (SD-Cold). Animals subjected to prolonged SD-Cold had smaller white adipocytes and beige-like cells within subcutaneous inguinal WAT (iWAT). Time-course analysis of gene expression with RNA-sequencing and quantitative PCR demonstrated that the mRNA expression of not only genes involved in lipid catabolism (lipolysis and beta-oxidation) but also lipid anabolism (lipogenesis and lipid desaturation) was simultaneously up-regulated prior to hibernation onset in the animals. The enhanced capacity of both lipid catabolism and lipid anabolism during hibernation period (HIB) is striking contrast to previous observations in fat-storing hibernators that only enhance catabolism during HIB. The mRNA expression of mTORC1 and PPAR signaling molecules increased, and pharmacological activation of PPARs indeed up-regulated lipid metabolism genes in iWAT explants from Syrian hamsters. These results suggest that the Syrian hamster rewires lipid metabolisms while preparing for hibernation to effectively utilize body fat and synthesize it from food intake during HIB. Frontiers Media S.A. 2019-01-28 /pmc/articles/PMC6360343/ /pubmed/30745884 http://dx.doi.org/10.3389/fphys.2018.01973 Text en Copyright © 2019 Chayama, Ando, Sato, Shigenobu, Anegawa, Fujimoto, Taii, Tamura, Miura and Yamaguchi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chayama, Yuichi
Ando, Lisa
Sato, Yuya
Shigenobu, Shuji
Anegawa, Daisuke
Fujimoto, Takayuki
Taii, Hiroki
Tamura, Yutaka
Miura, Masayuki
Yamaguchi, Yoshifumi
Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator
title Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator
title_full Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator
title_fullStr Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator
title_full_unstemmed Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator
title_short Molecular Basis of White Adipose Tissue Remodeling That Precedes and Coincides With Hibernation in the Syrian Hamster, a Food-Storing Hibernator
title_sort molecular basis of white adipose tissue remodeling that precedes and coincides with hibernation in the syrian hamster, a food-storing hibernator
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360343/
https://www.ncbi.nlm.nih.gov/pubmed/30745884
http://dx.doi.org/10.3389/fphys.2018.01973
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