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Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis
Papillary thyroid carcinoma (PTC) is the most commonly diagnosed endocrine cancer, and those with BRAF(V600E) mutation have high recurrence rate and less favorable clinical behavior. Genistein having anti-carcinoma effects in various types of carcinomas as an estrogen analog, but the mechanism of Ge...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360420/ https://www.ncbi.nlm.nih.gov/pubmed/30719173 http://dx.doi.org/10.7150/jca.28111 |
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author | Zhang, Chunyan Lv, Bin Yi, Cuihua Cui, Xiujie Sui, Shaofeng Li, Xueen Qi, Mei Hao, Chunyan Han, Bo Liu, Zhiyan |
author_facet | Zhang, Chunyan Lv, Bin Yi, Cuihua Cui, Xiujie Sui, Shaofeng Li, Xueen Qi, Mei Hao, Chunyan Han, Bo Liu, Zhiyan |
author_sort | Zhang, Chunyan |
collection | PubMed |
description | Papillary thyroid carcinoma (PTC) is the most commonly diagnosed endocrine cancer, and those with BRAF(V600E) mutation have high recurrence rate and less favorable clinical behavior. Genistein having anti-carcinoma effects in various types of carcinomas as an estrogen analog, but the mechanism of Genistein in the progression of PTC remains unknown. Genistein significantly inhibits the proliferation and the invasion (P < 0.01), and the apoptosis (P < 0.001) of all tumor cell lines, which was probably due to the inducing of the arrest in G2/M phase of the cell cycle (P < 0.001). The anti-proliferation and apoptosis inducing effects are more obvious in BCPAP, IHH4 cell lines harboring BRAF(V600E) mutation. Genistein significantly decreased the invasion of PTC cell lines and partially reverses epithelial mesenchymal transition in PTC cell lines. Functional study indicated that small interfering RNA (siRNA) knockdown of β-catenin significantly reverses the effect of genistein on EMT at protein levels. In conclusion, for the first time, our study suggested that genistein has anticarcinoma effect for PTC patients in the range of 2.5 and 80 μg/ml in thyroid carcinoma cells, which was probably through cytoplasmic translocation of β-catenin. Further study will be needed to determine whether genistein could be used in clinical trial of high-risk PTC. |
format | Online Article Text |
id | pubmed-6360420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-63604202019-02-04 Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis Zhang, Chunyan Lv, Bin Yi, Cuihua Cui, Xiujie Sui, Shaofeng Li, Xueen Qi, Mei Hao, Chunyan Han, Bo Liu, Zhiyan J Cancer Research Paper Papillary thyroid carcinoma (PTC) is the most commonly diagnosed endocrine cancer, and those with BRAF(V600E) mutation have high recurrence rate and less favorable clinical behavior. Genistein having anti-carcinoma effects in various types of carcinomas as an estrogen analog, but the mechanism of Genistein in the progression of PTC remains unknown. Genistein significantly inhibits the proliferation and the invasion (P < 0.01), and the apoptosis (P < 0.001) of all tumor cell lines, which was probably due to the inducing of the arrest in G2/M phase of the cell cycle (P < 0.001). The anti-proliferation and apoptosis inducing effects are more obvious in BCPAP, IHH4 cell lines harboring BRAF(V600E) mutation. Genistein significantly decreased the invasion of PTC cell lines and partially reverses epithelial mesenchymal transition in PTC cell lines. Functional study indicated that small interfering RNA (siRNA) knockdown of β-catenin significantly reverses the effect of genistein on EMT at protein levels. In conclusion, for the first time, our study suggested that genistein has anticarcinoma effect for PTC patients in the range of 2.5 and 80 μg/ml in thyroid carcinoma cells, which was probably through cytoplasmic translocation of β-catenin. Further study will be needed to determine whether genistein could be used in clinical trial of high-risk PTC. Ivyspring International Publisher 2019-01-01 /pmc/articles/PMC6360420/ /pubmed/30719173 http://dx.doi.org/10.7150/jca.28111 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Zhang, Chunyan Lv, Bin Yi, Cuihua Cui, Xiujie Sui, Shaofeng Li, Xueen Qi, Mei Hao, Chunyan Han, Bo Liu, Zhiyan Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis |
title | Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis |
title_full | Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis |
title_fullStr | Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis |
title_full_unstemmed | Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis |
title_short | Genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis |
title_sort | genistein inhibits human papillary thyroid cancer cell detachment, invasion and metastasis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360420/ https://www.ncbi.nlm.nih.gov/pubmed/30719173 http://dx.doi.org/10.7150/jca.28111 |
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