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Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype

INTRODUCTION: Many genetic studies are conducted on Obsessive-Compulsive Disorder (OCD). however, a high-throughput examination of proteome profile of this severe disease has not been performed yet. METHODS: Here, the proteomic study of OCD patients’ serum samples was conducted by the application of...

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Autores principales: Zamanian-Azodi, Mona, Rezaei-Tavirani, Mostafa, Mahboubi, Mohammad, Hamidpour, Mohsen, Rezaei Tavirani, Majid, Hamdieh, Mostafa, Rostami-Nejad, Mohammad, Nejadi, Naser, Derakhshan, Mohammad Kamran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Neuroscience Society 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360490/
https://www.ncbi.nlm.nih.gov/pubmed/30719248
http://dx.doi.org/10.32598/bcn.9.5.337
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author Zamanian-Azodi, Mona
Rezaei-Tavirani, Mostafa
Mahboubi, Mohammad
Hamidpour, Mohsen
Rezaei Tavirani, Majid
Hamdieh, Mostafa
Rostami-Nejad, Mohammad
Nejadi, Naser
Derakhshan, Mohammad Kamran
author_facet Zamanian-Azodi, Mona
Rezaei-Tavirani, Mostafa
Mahboubi, Mohammad
Hamidpour, Mohsen
Rezaei Tavirani, Majid
Hamdieh, Mostafa
Rostami-Nejad, Mohammad
Nejadi, Naser
Derakhshan, Mohammad Kamran
author_sort Zamanian-Azodi, Mona
collection PubMed
description INTRODUCTION: Many genetic studies are conducted on Obsessive-Compulsive Disorder (OCD). however, a high-throughput examination of proteome profile of this severe disease has not been performed yet. METHODS: Here, the proteomic study of OCD patients’ serum samples was conducted by the application of Two-Dimensional Electrophoresis (2DE) followed by Mass Spectrometry (MALDI-TOF-TOF). RESULTS: A total of 240 protein spots were detected and among them, five significant differentially expressed protein spots with the fold change of ≥1.5 were considered for further evaluations. These proteins include IGKC, GC, HPX, and two isoforms of HP. While IGKC and HP show down-regulation, GC and HPX indicate up-regulation. Moreover, a validation study of overall HP levels in patients’ serum via nephelometric quantification confirmed the lower levels of this protein in the serum of OCD patients. Additionally, enrichment analysis and validation test revealed that inflammation is one of most dominant processes in OCD. CONCLUSION: It is suggested that these candidate proteins and their underlying processes (especially, inflammation) may be linked to OCD pathophysiology and can promise a clinical use after extensive validation studies.
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spelling pubmed-63604902019-02-04 Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype Zamanian-Azodi, Mona Rezaei-Tavirani, Mostafa Mahboubi, Mohammad Hamidpour, Mohsen Rezaei Tavirani, Majid Hamdieh, Mostafa Rostami-Nejad, Mohammad Nejadi, Naser Derakhshan, Mohammad Kamran Basic Clin Neurosci Research Paper INTRODUCTION: Many genetic studies are conducted on Obsessive-Compulsive Disorder (OCD). however, a high-throughput examination of proteome profile of this severe disease has not been performed yet. METHODS: Here, the proteomic study of OCD patients’ serum samples was conducted by the application of Two-Dimensional Electrophoresis (2DE) followed by Mass Spectrometry (MALDI-TOF-TOF). RESULTS: A total of 240 protein spots were detected and among them, five significant differentially expressed protein spots with the fold change of ≥1.5 were considered for further evaluations. These proteins include IGKC, GC, HPX, and two isoforms of HP. While IGKC and HP show down-regulation, GC and HPX indicate up-regulation. Moreover, a validation study of overall HP levels in patients’ serum via nephelometric quantification confirmed the lower levels of this protein in the serum of OCD patients. Additionally, enrichment analysis and validation test revealed that inflammation is one of most dominant processes in OCD. CONCLUSION: It is suggested that these candidate proteins and their underlying processes (especially, inflammation) may be linked to OCD pathophysiology and can promise a clinical use after extensive validation studies. Iranian Neuroscience Society 2018 2018-09-01 /pmc/articles/PMC6360490/ /pubmed/30719248 http://dx.doi.org/10.32598/bcn.9.5.337 Text en Copyright© 2018 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zamanian-Azodi, Mona
Rezaei-Tavirani, Mostafa
Mahboubi, Mohammad
Hamidpour, Mohsen
Rezaei Tavirani, Majid
Hamdieh, Mostafa
Rostami-Nejad, Mohammad
Nejadi, Naser
Derakhshan, Mohammad Kamran
Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype
title Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype
title_full Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype
title_fullStr Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype
title_full_unstemmed Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype
title_short Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype
title_sort serum proteomic study of women with obsessive-compulsive disorder, washing subtype
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360490/
https://www.ncbi.nlm.nih.gov/pubmed/30719248
http://dx.doi.org/10.32598/bcn.9.5.337
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