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Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings

Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in developing countries including India. More importantly, Fusarium and Aspergillus are reported commonly implicating corneal ulcer and against this background the present work...

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Autores principales: Manikandan, Palanisamy, Abdel-hadi, Ahmed, Randhir Babu Singh, Yendrembam, Revathi, Rajaraman, Anita, Raghavan, Banawas, Saeed, Bin Dukhyil, Abdul Aziz, Alshehri, Bader, Shobana, Coimbatore Subramanian, Panneer Selvam, Kanesan, Narendran, Venkatapathy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360544/
https://www.ncbi.nlm.nih.gov/pubmed/30800674
http://dx.doi.org/10.1155/2019/6395840
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author Manikandan, Palanisamy
Abdel-hadi, Ahmed
Randhir Babu Singh, Yendrembam
Revathi, Rajaraman
Anita, Raghavan
Banawas, Saeed
Bin Dukhyil, Abdul Aziz
Alshehri, Bader
Shobana, Coimbatore Subramanian
Panneer Selvam, Kanesan
Narendran, Venkatapathy
author_facet Manikandan, Palanisamy
Abdel-hadi, Ahmed
Randhir Babu Singh, Yendrembam
Revathi, Rajaraman
Anita, Raghavan
Banawas, Saeed
Bin Dukhyil, Abdul Aziz
Alshehri, Bader
Shobana, Coimbatore Subramanian
Panneer Selvam, Kanesan
Narendran, Venkatapathy
author_sort Manikandan, Palanisamy
collection PubMed
description Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in developing countries including India. More importantly, Fusarium and Aspergillus are reported commonly implicating corneal ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the two fungal keratitis. During the project period, a total of 500 corneal scrapings were collected from suspected mycotic keratitis patients, of which 411 (82.2%) were culture positive for bacteria, fungi, and parasites. Among fungal aetiologies, Fusarium (216, 52.5% of 411) and Aspergillus (68, 16.5% of 411) were predominantly determined. While the study revealed a male preponderance with both the fungal keratitis , it further brought out that polyene compounds (natamycin and amphotericin B) and azoles were active, respectively, against Fusarium spp. and Aspergillus spp. Additionally, 94.1% of culture proven Fusarium keratitis and, respectively, 100% and 63.6% of A. flavus and A. fumigatus were confirmed by multiplex PCR. The sensitivity of the PCR employed in the present study was noted to be 10 fg/μl, 1 pg/μl, and 300 pg/μl of DNA, respectively, for Fusarium, A. flavus, and A. fumigatus. Alarming fact was that Fusarium and Aspergillus regionally remained to be the common cause of mycotic keratitis and the Fusarium isolates had a higher antifungal resistance than Aspergillus strains against most of the test drugs.
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spelling pubmed-63605442019-02-24 Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings Manikandan, Palanisamy Abdel-hadi, Ahmed Randhir Babu Singh, Yendrembam Revathi, Rajaraman Anita, Raghavan Banawas, Saeed Bin Dukhyil, Abdul Aziz Alshehri, Bader Shobana, Coimbatore Subramanian Panneer Selvam, Kanesan Narendran, Venkatapathy Biomed Res Int Research Article Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in developing countries including India. More importantly, Fusarium and Aspergillus are reported commonly implicating corneal ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the two fungal keratitis. During the project period, a total of 500 corneal scrapings were collected from suspected mycotic keratitis patients, of which 411 (82.2%) were culture positive for bacteria, fungi, and parasites. Among fungal aetiologies, Fusarium (216, 52.5% of 411) and Aspergillus (68, 16.5% of 411) were predominantly determined. While the study revealed a male preponderance with both the fungal keratitis , it further brought out that polyene compounds (natamycin and amphotericin B) and azoles were active, respectively, against Fusarium spp. and Aspergillus spp. Additionally, 94.1% of culture proven Fusarium keratitis and, respectively, 100% and 63.6% of A. flavus and A. fumigatus were confirmed by multiplex PCR. The sensitivity of the PCR employed in the present study was noted to be 10 fg/μl, 1 pg/μl, and 300 pg/μl of DNA, respectively, for Fusarium, A. flavus, and A. fumigatus. Alarming fact was that Fusarium and Aspergillus regionally remained to be the common cause of mycotic keratitis and the Fusarium isolates had a higher antifungal resistance than Aspergillus strains against most of the test drugs. Hindawi 2019-01-20 /pmc/articles/PMC6360544/ /pubmed/30800674 http://dx.doi.org/10.1155/2019/6395840 Text en Copyright © 2019 Palanisamy Manikandan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Manikandan, Palanisamy
Abdel-hadi, Ahmed
Randhir Babu Singh, Yendrembam
Revathi, Rajaraman
Anita, Raghavan
Banawas, Saeed
Bin Dukhyil, Abdul Aziz
Alshehri, Bader
Shobana, Coimbatore Subramanian
Panneer Selvam, Kanesan
Narendran, Venkatapathy
Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings
title Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings
title_full Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings
title_fullStr Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings
title_full_unstemmed Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings
title_short Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings
title_sort fungal keratitis: epidemiology, rapid detection, and antifungal susceptibilities of fusarium and aspergillus isolates from corneal scrapings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360544/
https://www.ncbi.nlm.nih.gov/pubmed/30800674
http://dx.doi.org/10.1155/2019/6395840
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