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Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2

Oxidative stress may arise from a variety of pathologies and results in the formation of toxic and reactive chemical species. Extensive research has been done to establish mechanisms of formation and cytotoxic effects of a number of different products of oxidation stress including peroxynitrite (PN)...

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Autores principales: Verenich, Svetlana, Gerk, Phillip M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360629/
https://www.ncbi.nlm.nih.gov/pubmed/30800667
http://dx.doi.org/10.1155/2019/3891535
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author Verenich, Svetlana
Gerk, Phillip M.
author_facet Verenich, Svetlana
Gerk, Phillip M.
author_sort Verenich, Svetlana
collection PubMed
description Oxidative stress may arise from a variety of pathologies and results in the formation of toxic and reactive chemical species. Extensive research has been done to establish mechanisms of formation and cytotoxic effects of a number of different products of oxidation stress including peroxynitrite (PN) and 4-hydroxynonenal (4HNE). However, relatively few studies have investigated their effects on ATP-binding cassette (ABC) transporters. The objective of this investigation was to determine the effects of PN and 4HNE on BCRP/ABCG2. To eliminate the effect of metabolic enzymes, the experiments were carried out with inside-out Sf9 membrane vesicles overexpressing BCRP/ABCG2 using riboflavin as a substrate. The experiments revealed that PN produced IC(50) of about 31.2 ± 2.7 μM, based upon initial concentrations. The IC(50) for 4HNE was estimated to be 92 ± 1.4 μM. Preincubation of membrane vesicles with either PN or 4HNE caused the maximal rate of transport (V(max)) to drop drastically, up to 19 times, with no or much smaller effect on K(m). Thus, PN and 4NE can inhibit BCRP transport activity.
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spelling pubmed-63606292019-02-24 Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2 Verenich, Svetlana Gerk, Phillip M. Biomed Res Int Research Article Oxidative stress may arise from a variety of pathologies and results in the formation of toxic and reactive chemical species. Extensive research has been done to establish mechanisms of formation and cytotoxic effects of a number of different products of oxidation stress including peroxynitrite (PN) and 4-hydroxynonenal (4HNE). However, relatively few studies have investigated their effects on ATP-binding cassette (ABC) transporters. The objective of this investigation was to determine the effects of PN and 4HNE on BCRP/ABCG2. To eliminate the effect of metabolic enzymes, the experiments were carried out with inside-out Sf9 membrane vesicles overexpressing BCRP/ABCG2 using riboflavin as a substrate. The experiments revealed that PN produced IC(50) of about 31.2 ± 2.7 μM, based upon initial concentrations. The IC(50) for 4HNE was estimated to be 92 ± 1.4 μM. Preincubation of membrane vesicles with either PN or 4HNE caused the maximal rate of transport (V(max)) to drop drastically, up to 19 times, with no or much smaller effect on K(m). Thus, PN and 4NE can inhibit BCRP transport activity. Hindawi 2019-01-21 /pmc/articles/PMC6360629/ /pubmed/30800667 http://dx.doi.org/10.1155/2019/3891535 Text en Copyright © 2019 Svetlana Verenich and Phillip M. Gerk. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Verenich, Svetlana
Gerk, Phillip M.
Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2
title Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2
title_full Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2
title_fullStr Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2
title_full_unstemmed Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2
title_short Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2
title_sort peroxynitrite and 4-hydroxynonenal inactivate breast cancer resistance protein/abcg2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360629/
https://www.ncbi.nlm.nih.gov/pubmed/30800667
http://dx.doi.org/10.1155/2019/3891535
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