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Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis

BACKGROUND: Due to its wide range of applications in the food, pharmaceutical and chemical fields, microbial synthesis of succinic acid is receiving growing attention, generating already relevant industrial results, as well as fueling constant research for improvements. In order to develop a sustain...

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Autores principales: Cimini, Donatella, Zaccariello, Lucio, D’Ambrosio, Sergio, Lama, Licia, Ruoppolo, Giovanna, Pepe, Olimpia, Faraco, Vincenza, Schiraldi, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360672/
https://www.ncbi.nlm.nih.gov/pubmed/30740143
http://dx.doi.org/10.1186/s13068-019-1362-6
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author Cimini, Donatella
Zaccariello, Lucio
D’Ambrosio, Sergio
Lama, Licia
Ruoppolo, Giovanna
Pepe, Olimpia
Faraco, Vincenza
Schiraldi, Chiara
author_facet Cimini, Donatella
Zaccariello, Lucio
D’Ambrosio, Sergio
Lama, Licia
Ruoppolo, Giovanna
Pepe, Olimpia
Faraco, Vincenza
Schiraldi, Chiara
author_sort Cimini, Donatella
collection PubMed
description BACKGROUND: Due to its wide range of applications in the food, pharmaceutical and chemical fields, microbial synthesis of succinic acid is receiving growing attention, generating already relevant industrial results, as well as fueling constant research for improvements. In order to develop a sustainable process, a special focus is now set on the exploitation and conversion of lignocellulosic biomasses into platform chemicals. RESULTS: In the present work we used Basfia succiniciproducens BPP7 in separated hydrolysis and fermentation experiments with Arundo donax as starting material. Fed-batch strategies showed a maximal production of about 37 g/L of succinic acid after 43 h of growth and a productivity of 0.9 g/L h on the pilot scale. Global mass balance calculations demonstrated a hydrolysis and fermentation efficiency of about 75%. Moreover, the application of a material flow analysis showed the obtainment of 88.5 and 52 % of succinic acid, per kg of virgin biomass and on the total generated output, respectively. CONCLUSIONS: The use of fed-batch strategies for the growth of B. succiniciproducens on A. donax improved the titer and productivity of succinic acid on pre-pilot scale. Process evaluation through material flow analysis showed successful results and predicted a yield of succinic acid of about 30% in a fed-batch process that uses A. donax as only carbon source also in the feed. Preliminary considerations on the possibility to achieve an energetic valorization of the residual solid coming from the fermentation process were also carried out. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-019-1362-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-63606722019-02-08 Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis Cimini, Donatella Zaccariello, Lucio D’Ambrosio, Sergio Lama, Licia Ruoppolo, Giovanna Pepe, Olimpia Faraco, Vincenza Schiraldi, Chiara Biotechnol Biofuels Research BACKGROUND: Due to its wide range of applications in the food, pharmaceutical and chemical fields, microbial synthesis of succinic acid is receiving growing attention, generating already relevant industrial results, as well as fueling constant research for improvements. In order to develop a sustainable process, a special focus is now set on the exploitation and conversion of lignocellulosic biomasses into platform chemicals. RESULTS: In the present work we used Basfia succiniciproducens BPP7 in separated hydrolysis and fermentation experiments with Arundo donax as starting material. Fed-batch strategies showed a maximal production of about 37 g/L of succinic acid after 43 h of growth and a productivity of 0.9 g/L h on the pilot scale. Global mass balance calculations demonstrated a hydrolysis and fermentation efficiency of about 75%. Moreover, the application of a material flow analysis showed the obtainment of 88.5 and 52 % of succinic acid, per kg of virgin biomass and on the total generated output, respectively. CONCLUSIONS: The use of fed-batch strategies for the growth of B. succiniciproducens on A. donax improved the titer and productivity of succinic acid on pre-pilot scale. Process evaluation through material flow analysis showed successful results and predicted a yield of succinic acid of about 30% in a fed-batch process that uses A. donax as only carbon source also in the feed. Preliminary considerations on the possibility to achieve an energetic valorization of the residual solid coming from the fermentation process were also carried out. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-019-1362-6) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-04 /pmc/articles/PMC6360672/ /pubmed/30740143 http://dx.doi.org/10.1186/s13068-019-1362-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Cimini, Donatella
Zaccariello, Lucio
D’Ambrosio, Sergio
Lama, Licia
Ruoppolo, Giovanna
Pepe, Olimpia
Faraco, Vincenza
Schiraldi, Chiara
Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
title Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
title_full Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
title_fullStr Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
title_full_unstemmed Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
title_short Improved production of succinic acid from Basfia succiniciproducens growing on A. donax and process evaluation through material flow analysis
title_sort improved production of succinic acid from basfia succiniciproducens growing on a. donax and process evaluation through material flow analysis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360672/
https://www.ncbi.nlm.nih.gov/pubmed/30740143
http://dx.doi.org/10.1186/s13068-019-1362-6
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