PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin

Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be deve...

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Autores principales: Furusawa, Yoshikazu, Yamada, Shinji, Itai, Shunsuke, Nakamura, Takuro, Yanaka, Miyuki, Sano, Masato, Harada, Hiroyuki, Fukui, Masato, Kaneko, Mika K., Kato, Yukinari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360987/
https://www.ncbi.nlm.nih.gov/pubmed/30766925
http://dx.doi.org/10.1016/j.bbrep.2019.01.009
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author Furusawa, Yoshikazu
Yamada, Shinji
Itai, Shunsuke
Nakamura, Takuro
Yanaka, Miyuki
Sano, Masato
Harada, Hiroyuki
Fukui, Masato
Kaneko, Mika K.
Kato, Yukinari
author_facet Furusawa, Yoshikazu
Yamada, Shinji
Itai, Shunsuke
Nakamura, Takuro
Yanaka, Miyuki
Sano, Masato
Harada, Hiroyuki
Fukui, Masato
Kaneko, Mika K.
Kato, Yukinari
author_sort Furusawa, Yoshikazu
collection PubMed
description Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG(2a), kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting. Furthermore, PMab-219 strongly stained CHO/horPDPN via immunohistochemistry. These findings suggest that PMab-219 is useful for investigating the function of horPDPN.
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spelling pubmed-63609872019-02-14 PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin Furusawa, Yoshikazu Yamada, Shinji Itai, Shunsuke Nakamura, Takuro Yanaka, Miyuki Sano, Masato Harada, Hiroyuki Fukui, Masato Kaneko, Mika K. Kato, Yukinari Biochem Biophys Rep Research Article Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG(2a), kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting. Furthermore, PMab-219 strongly stained CHO/horPDPN via immunohistochemistry. These findings suggest that PMab-219 is useful for investigating the function of horPDPN. Elsevier 2019-02-02 /pmc/articles/PMC6360987/ /pubmed/30766925 http://dx.doi.org/10.1016/j.bbrep.2019.01.009 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Furusawa, Yoshikazu
Yamada, Shinji
Itai, Shunsuke
Nakamura, Takuro
Yanaka, Miyuki
Sano, Masato
Harada, Hiroyuki
Fukui, Masato
Kaneko, Mika K.
Kato, Yukinari
PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_full PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_fullStr PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_full_unstemmed PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_short PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_sort pmab-219: a monoclonal antibody for the immunohistochemical analysis of horse podoplanin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360987/
https://www.ncbi.nlm.nih.gov/pubmed/30766925
http://dx.doi.org/10.1016/j.bbrep.2019.01.009
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