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Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans

Cell-fate maintenance is important to preserve the variety of cell types that are essential for the formation and function of tissues. We previously showed that the acetylated histone-binding protein BET-1 maintains cell fate by recruiting the histone variant H2A.z. Here, we report that Caenorhabdit...

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Autores principales: Shibata, Yukimasa, Seki, Yoshiyuki, Nishiwaki, Kiyoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361200/
https://www.ncbi.nlm.nih.gov/pubmed/30635266
http://dx.doi.org/10.1242/bio.038448
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author Shibata, Yukimasa
Seki, Yoshiyuki
Nishiwaki, Kiyoji
author_facet Shibata, Yukimasa
Seki, Yoshiyuki
Nishiwaki, Kiyoji
author_sort Shibata, Yukimasa
collection PubMed
description Cell-fate maintenance is important to preserve the variety of cell types that are essential for the formation and function of tissues. We previously showed that the acetylated histone-binding protein BET-1 maintains cell fate by recruiting the histone variant H2A.z. Here, we report that Caenorhabditis elegans TLK-1 and the histone H3 chaperone CAF1 prevent the accumulation of histone variant H3.3. In addition, TLK-1 and CAF1 maintain cell fate by repressing ectopic expression of transcription factors that induce cell-fate specification. Genetic analyses suggested that TLK-1 and BET-1 act in parallel pathways. In tlk-1 mutants, the loss of SIN-3, which promotes histone acetylation, suppressed a defect in cell-fate maintenance in a manner dependent on MYST family histone acetyltransferase MYS-2 and BET-1. sin-3 mutation also suppressed abnormal H3.3 incorporation. Thus, we propose a hypothesis that the regulation and interaction of histone variants play crucial roles in cell-fate maintenance through the regulation of selector genes.
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spelling pubmed-63612002019-02-05 Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans Shibata, Yukimasa Seki, Yoshiyuki Nishiwaki, Kiyoji Biol Open Research Article Cell-fate maintenance is important to preserve the variety of cell types that are essential for the formation and function of tissues. We previously showed that the acetylated histone-binding protein BET-1 maintains cell fate by recruiting the histone variant H2A.z. Here, we report that Caenorhabditis elegans TLK-1 and the histone H3 chaperone CAF1 prevent the accumulation of histone variant H3.3. In addition, TLK-1 and CAF1 maintain cell fate by repressing ectopic expression of transcription factors that induce cell-fate specification. Genetic analyses suggested that TLK-1 and BET-1 act in parallel pathways. In tlk-1 mutants, the loss of SIN-3, which promotes histone acetylation, suppressed a defect in cell-fate maintenance in a manner dependent on MYST family histone acetyltransferase MYS-2 and BET-1. sin-3 mutation also suppressed abnormal H3.3 incorporation. Thus, we propose a hypothesis that the regulation and interaction of histone variants play crucial roles in cell-fate maintenance through the regulation of selector genes. The Company of Biologists Ltd 2019-01-11 /pmc/articles/PMC6361200/ /pubmed/30635266 http://dx.doi.org/10.1242/bio.038448 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Shibata, Yukimasa
Seki, Yoshiyuki
Nishiwaki, Kiyoji
Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans
title Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans
title_full Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans
title_fullStr Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans
title_full_unstemmed Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans
title_short Maintenance of cell fates and regulation of the histone variant H3.3 by TLK kinase in Caenorhabditis elegans
title_sort maintenance of cell fates and regulation of the histone variant h3.3 by tlk kinase in caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361200/
https://www.ncbi.nlm.nih.gov/pubmed/30635266
http://dx.doi.org/10.1242/bio.038448
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