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Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney

A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 ti...

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Autores principales: Chen, Bin, Zheng, Zaiyu, Yang, Jinxian, Chi, Hongshu, Huang, He, Gong, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361207/
https://www.ncbi.nlm.nih.gov/pubmed/30429125
http://dx.doi.org/10.1242/bio.037507
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author Chen, Bin
Zheng, Zaiyu
Yang, Jinxian
Chi, Hongshu
Huang, He
Gong, Hui
author_facet Chen, Bin
Zheng, Zaiyu
Yang, Jinxian
Chi, Hongshu
Huang, He
Gong, Hui
author_sort Chen, Bin
collection PubMed
description A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 times. This cell line consists predominantly of fibroblast-like cells, and can grow at 15–37°C under an optimum temperature of 26°C. The origin of this cell line was confirmed by polymerase chain reaction (PCR) amplification and 18s recombinant (r)RNA sequencing. The chromosome analysis of EK cells at passage 58 revealed an ananeuploid karyotype. The EK cells were successfully transfected with the Pegfp-N1 plasmid, suggesting its potential in genetic studies. The susceptibility test showed a significant cytopathic effect (CPE) in EK cells for Rana grylio virus, and the viral replication was evidenced with quantitative real-time PCR (qRT-PCR) assay. After poly (I:C) stimulation, the expression of the immune-related molecules including interferon regulatory factor-3 (irf3), interferon regulatory factor-7 (irf7) and cytochrome P450 (CYP450) were significantly upregulated in EK cells, while the expression of transforming growth factor (TGF-β) was downregulated. These results suggested the potential of EK cell line as a model in gene engineering, virus identification and environmental toxicology.
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spelling pubmed-63612072019-02-05 Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney Chen, Bin Zheng, Zaiyu Yang, Jinxian Chi, Hongshu Huang, He Gong, Hui Biol Open Research Article A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 times. This cell line consists predominantly of fibroblast-like cells, and can grow at 15–37°C under an optimum temperature of 26°C. The origin of this cell line was confirmed by polymerase chain reaction (PCR) amplification and 18s recombinant (r)RNA sequencing. The chromosome analysis of EK cells at passage 58 revealed an ananeuploid karyotype. The EK cells were successfully transfected with the Pegfp-N1 plasmid, suggesting its potential in genetic studies. The susceptibility test showed a significant cytopathic effect (CPE) in EK cells for Rana grylio virus, and the viral replication was evidenced with quantitative real-time PCR (qRT-PCR) assay. After poly (I:C) stimulation, the expression of the immune-related molecules including interferon regulatory factor-3 (irf3), interferon regulatory factor-7 (irf7) and cytochrome P450 (CYP450) were significantly upregulated in EK cells, while the expression of transforming growth factor (TGF-β) was downregulated. These results suggested the potential of EK cell line as a model in gene engineering, virus identification and environmental toxicology. The Company of Biologists Ltd 2018-11-14 /pmc/articles/PMC6361207/ /pubmed/30429125 http://dx.doi.org/10.1242/bio.037507 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Chen, Bin
Zheng, Zaiyu
Yang, Jinxian
Chi, Hongshu
Huang, He
Gong, Hui
Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
title Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
title_full Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
title_fullStr Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
title_full_unstemmed Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
title_short Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
title_sort development and characterization of a new cell line derived from european eel anguilla anguilla kidney
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361207/
https://www.ncbi.nlm.nih.gov/pubmed/30429125
http://dx.doi.org/10.1242/bio.037507
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