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Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine

Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment media. Our results sh...

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Autores principales: Kim, Sang Hwan, Lee, Ji Hye, Yoon, Jong Taek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361312/
https://www.ncbi.nlm.nih.gov/pubmed/30729068
http://dx.doi.org/10.7717/peerj.6344
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author Kim, Sang Hwan
Lee, Ji Hye
Yoon, Jong Taek
author_facet Kim, Sang Hwan
Lee, Ji Hye
Yoon, Jong Taek
author_sort Kim, Sang Hwan
collection PubMed
description Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment media. Our results show that the activity of MMP-2 gelatinase was higher in the CL2 and CL1 of luteal phase, was gradually decreased in the CH2 and CH3 of luteal phase. In particular, the expressions of P4-r and survival-associated genes (IGFr, PI3K, AKT, and mTOR) were strongly induced during CL3 stage, whereas the levels of these genes in corpus luteum (CL) were lower during CL2 and CL1 stages. In the cultured lutein cells analyzed, we found that as MMPs increase, genes related to apoptosis (20α-hydroxy steroid dehydrogenase and caspase-3) also increase. In other words, the results for P4-r and survival-related gene expression patterns in the luteal cells were contrary to the MMPs activation results. These results indicate that active MMPs are differentially expressed to induce the expression of genes associated with programed cell death from the degrading luteal cells. Therefore, our results suggest that the MMPs activation may lead to luteal cell development or death.
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spelling pubmed-63613122019-02-06 Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine Kim, Sang Hwan Lee, Ji Hye Yoon, Jong Taek PeerJ Agricultural Science Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment media. Our results show that the activity of MMP-2 gelatinase was higher in the CL2 and CL1 of luteal phase, was gradually decreased in the CH2 and CH3 of luteal phase. In particular, the expressions of P4-r and survival-associated genes (IGFr, PI3K, AKT, and mTOR) were strongly induced during CL3 stage, whereas the levels of these genes in corpus luteum (CL) were lower during CL2 and CL1 stages. In the cultured lutein cells analyzed, we found that as MMPs increase, genes related to apoptosis (20α-hydroxy steroid dehydrogenase and caspase-3) also increase. In other words, the results for P4-r and survival-related gene expression patterns in the luteal cells were contrary to the MMPs activation results. These results indicate that active MMPs are differentially expressed to induce the expression of genes associated with programed cell death from the degrading luteal cells. Therefore, our results suggest that the MMPs activation may lead to luteal cell development or death. PeerJ Inc. 2019-01-30 /pmc/articles/PMC6361312/ /pubmed/30729068 http://dx.doi.org/10.7717/peerj.6344 Text en © 2019 Kim et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Kim, Sang Hwan
Lee, Ji Hye
Yoon, Jong Taek
Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
title Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
title_full Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
title_fullStr Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
title_full_unstemmed Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
title_short Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
title_sort expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361312/
https://www.ncbi.nlm.nih.gov/pubmed/30729068
http://dx.doi.org/10.7717/peerj.6344
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