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Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex

BACKGROUND: Intratumoral injection is a palliative treatment that aims at further improvement in the survival and quality of life of patients with advanced or recurrent carcinomas, or cancer patients with severe comorbidities or those with a poor performance status. METHODS: In this study, a solvent...

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Autores principales: Chu, Xiao-Yang, Huang, Wei, Wang, Yu-Li, Meng, Ling-Wei, Chen, Li-Qing, Jin, Ming-Ji, Chen, Lu, Gao, Chun-Hong, Ge, Cheng, Gao, Zhong-Gao, Gao, Chun-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361321/
https://www.ncbi.nlm.nih.gov/pubmed/30774330
http://dx.doi.org/10.2147/IJN.S188667
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author Chu, Xiao-Yang
Huang, Wei
Wang, Yu-Li
Meng, Ling-Wei
Chen, Li-Qing
Jin, Ming-Ji
Chen, Lu
Gao, Chun-Hong
Ge, Cheng
Gao, Zhong-Gao
Gao, Chun-Sheng
author_facet Chu, Xiao-Yang
Huang, Wei
Wang, Yu-Li
Meng, Ling-Wei
Chen, Li-Qing
Jin, Ming-Ji
Chen, Lu
Gao, Chun-Hong
Ge, Cheng
Gao, Zhong-Gao
Gao, Chun-Sheng
author_sort Chu, Xiao-Yang
collection PubMed
description BACKGROUND: Intratumoral injection is a palliative treatment that aims at further improvement in the survival and quality of life of patients with advanced or recurrent carcinomas, or cancer patients with severe comorbidities or those with a poor performance status. METHODS: In this study, a solvent-injection method was used to prepare paclitaxel–cholesterol complex-loaded lecithin–chitosan nanoparticles (PTX-CH-loaded LCS_NPs) for intratumoral injection therapy, and the physicochemical properties of NPs were well characterized. RESULTS: The particle size and zeta potential of PTX-CH-loaded LCS_NPs were 142.83±0.25 nm and 13.50±0.20 mV, respectively. Release behavior of PTX from PTX-CH-loaded LCS_NPs showed a pH-sensitive pattern. The result of cell uptake assay showed that PTX-CH-loaded LCS_NPs could effectively enter cells via the energy-dependent caveolae-mediated endocytosis and macropinocytosis in company with the Golgi apparatus. Meanwhile, PTX-CH-loaded LCS_NPs had a better ability to induce cell apoptosis than PTX solution. The in vivo antitumor results suggested that PTX-CH-loaded LCS_NPs effectively inhibited mouse mammary cancer growth and metastasis to distant organs and significantly improved the survival rate of tumor-bearing mice by intratumoral administration. CONCLUSION: In general, our study demonstrated that PTX-CH-loaded LCS_NPs used for palliative treatment by intratumoral injection showed improved safety and antitumor efficacy, which provided an alternative approach in the field of palliative chemotherapy.
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spelling pubmed-63613212019-02-15 Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex Chu, Xiao-Yang Huang, Wei Wang, Yu-Li Meng, Ling-Wei Chen, Li-Qing Jin, Ming-Ji Chen, Lu Gao, Chun-Hong Ge, Cheng Gao, Zhong-Gao Gao, Chun-Sheng Int J Nanomedicine Original Research BACKGROUND: Intratumoral injection is a palliative treatment that aims at further improvement in the survival and quality of life of patients with advanced or recurrent carcinomas, or cancer patients with severe comorbidities or those with a poor performance status. METHODS: In this study, a solvent-injection method was used to prepare paclitaxel–cholesterol complex-loaded lecithin–chitosan nanoparticles (PTX-CH-loaded LCS_NPs) for intratumoral injection therapy, and the physicochemical properties of NPs were well characterized. RESULTS: The particle size and zeta potential of PTX-CH-loaded LCS_NPs were 142.83±0.25 nm and 13.50±0.20 mV, respectively. Release behavior of PTX from PTX-CH-loaded LCS_NPs showed a pH-sensitive pattern. The result of cell uptake assay showed that PTX-CH-loaded LCS_NPs could effectively enter cells via the energy-dependent caveolae-mediated endocytosis and macropinocytosis in company with the Golgi apparatus. Meanwhile, PTX-CH-loaded LCS_NPs had a better ability to induce cell apoptosis than PTX solution. The in vivo antitumor results suggested that PTX-CH-loaded LCS_NPs effectively inhibited mouse mammary cancer growth and metastasis to distant organs and significantly improved the survival rate of tumor-bearing mice by intratumoral administration. CONCLUSION: In general, our study demonstrated that PTX-CH-loaded LCS_NPs used for palliative treatment by intratumoral injection showed improved safety and antitumor efficacy, which provided an alternative approach in the field of palliative chemotherapy. Dove Medical Press 2019-01-23 /pmc/articles/PMC6361321/ /pubmed/30774330 http://dx.doi.org/10.2147/IJN.S188667 Text en © 2019 Chu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Chu, Xiao-Yang
Huang, Wei
Wang, Yu-Li
Meng, Ling-Wei
Chen, Li-Qing
Jin, Ming-Ji
Chen, Lu
Gao, Chun-Hong
Ge, Cheng
Gao, Zhong-Gao
Gao, Chun-Sheng
Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex
title Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex
title_full Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex
title_fullStr Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex
title_full_unstemmed Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex
title_short Improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex
title_sort improving antitumor outcomes for palliative intratumoral injection therapy through lecithin– chitosan nanoparticles loading paclitaxel– cholesterol complex
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361321/
https://www.ncbi.nlm.nih.gov/pubmed/30774330
http://dx.doi.org/10.2147/IJN.S188667
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