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Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells
Recently, there has been increased attention on the analysis of circulating tumor cells (CTCs), also known as liquid biopsy, owing to its potential benefits in cancer diagnosis and treatment. Circulating tumor cells are released from primary tumor lesions into the blood stream and eventually metasta...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361580/ https://www.ncbi.nlm.nih.gov/pubmed/30549153 http://dx.doi.org/10.1111/cas.13915 |
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author | Abouleila, Yasmine Onidani, Kaoru Ali, Ahmed Shoji, Hirokazu Kawai, Takayuki Lim, Chwee Teck Kumar, Vipin Okaya, Shinobu Kato, Ken Hiyama, Eiso Yanagida, Toshio Masujima, Tsutomu Shimizu, Yoshihiro Honda, Kazufumi |
author_facet | Abouleila, Yasmine Onidani, Kaoru Ali, Ahmed Shoji, Hirokazu Kawai, Takayuki Lim, Chwee Teck Kumar, Vipin Okaya, Shinobu Kato, Ken Hiyama, Eiso Yanagida, Toshio Masujima, Tsutomu Shimizu, Yoshihiro Honda, Kazufumi |
author_sort | Abouleila, Yasmine |
collection | PubMed |
description | Recently, there has been increased attention on the analysis of circulating tumor cells (CTCs), also known as liquid biopsy, owing to its potential benefits in cancer diagnosis and treatment. Circulating tumor cells are released from primary tumor lesions into the blood stream and eventually metastasize to distant body organs. However, a major hurdle with CTC analysis is their natural scarcity. Existing methods lack sensitivity, specificity, or reproducibility required in CTC characterization and detection. Here, we report untargeted molecular profiling of single CTCs obtained from gastric cancer and colorectal cancer patients, using live single cell mass spectrometry integrated with microfluidics‐based cell enrichment techniques. Using this approach, we showed the difference in the metabolomic profile between CTCs originating from different cancer groups. Moreover, potential biomarkers were putatively annotated to be specific to each cancer type. |
format | Online Article Text |
id | pubmed-6361580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63615802019-02-14 Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells Abouleila, Yasmine Onidani, Kaoru Ali, Ahmed Shoji, Hirokazu Kawai, Takayuki Lim, Chwee Teck Kumar, Vipin Okaya, Shinobu Kato, Ken Hiyama, Eiso Yanagida, Toshio Masujima, Tsutomu Shimizu, Yoshihiro Honda, Kazufumi Cancer Sci Original Articles Recently, there has been increased attention on the analysis of circulating tumor cells (CTCs), also known as liquid biopsy, owing to its potential benefits in cancer diagnosis and treatment. Circulating tumor cells are released from primary tumor lesions into the blood stream and eventually metastasize to distant body organs. However, a major hurdle with CTC analysis is their natural scarcity. Existing methods lack sensitivity, specificity, or reproducibility required in CTC characterization and detection. Here, we report untargeted molecular profiling of single CTCs obtained from gastric cancer and colorectal cancer patients, using live single cell mass spectrometry integrated with microfluidics‐based cell enrichment techniques. Using this approach, we showed the difference in the metabolomic profile between CTCs originating from different cancer groups. Moreover, potential biomarkers were putatively annotated to be specific to each cancer type. John Wiley and Sons Inc. 2019-01-05 2019-02 /pmc/articles/PMC6361580/ /pubmed/30549153 http://dx.doi.org/10.1111/cas.13915 Text en © 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Abouleila, Yasmine Onidani, Kaoru Ali, Ahmed Shoji, Hirokazu Kawai, Takayuki Lim, Chwee Teck Kumar, Vipin Okaya, Shinobu Kato, Ken Hiyama, Eiso Yanagida, Toshio Masujima, Tsutomu Shimizu, Yoshihiro Honda, Kazufumi Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells |
title | Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells |
title_full | Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells |
title_fullStr | Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells |
title_full_unstemmed | Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells |
title_short | Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells |
title_sort | live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361580/ https://www.ncbi.nlm.nih.gov/pubmed/30549153 http://dx.doi.org/10.1111/cas.13915 |
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