Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR

A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5’-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By...

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Detalles Bibliográficos
Autores principales: GOTO, Yusuke, YAEGASHI, Gakuji, KUMAGAI, Yoshihiro, OGASAWARA, Fusae, GOTO, Makiko, MASE, Masaji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Avian Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361656/
https://www.ncbi.nlm.nih.gov/pubmed/30464075
http://dx.doi.org/10.1292/jvms.18-0550
Descripción
Sumario:A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5’-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By applying this method to the chicken samples with suspected AEV infection in Japan, we successfully obtained PCR products of the predicted size from all samples, and we confirmed the presence of AEV via sequence analysis.

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