Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR

A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5’-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By...

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Autores principales: GOTO, Yusuke, YAEGASHI, Gakuji, KUMAGAI, Yoshihiro, OGASAWARA, Fusae, GOTO, Makiko, MASE, Masaji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Avian Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361656/
https://www.ncbi.nlm.nih.gov/pubmed/30464075
http://dx.doi.org/10.1292/jvms.18-0550
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author GOTO, Yusuke
YAEGASHI, Gakuji
KUMAGAI, Yoshihiro
OGASAWARA, Fusae
GOTO, Makiko
MASE, Masaji
author_facet GOTO, Yusuke
YAEGASHI, Gakuji
KUMAGAI, Yoshihiro
OGASAWARA, Fusae
GOTO, Makiko
MASE, Masaji
author_sort GOTO, Yusuke
collection PubMed
description A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5’-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By applying this method to the chicken samples with suspected AEV infection in Japan, we successfully obtained PCR products of the predicted size from all samples, and we confirmed the presence of AEV via sequence analysis.
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spelling pubmed-63616562019-02-08 Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR GOTO, Yusuke YAEGASHI, Gakuji KUMAGAI, Yoshihiro OGASAWARA, Fusae GOTO, Makiko MASE, Masaji J Vet Med Sci Avian Pathology A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5’-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By applying this method to the chicken samples with suspected AEV infection in Japan, we successfully obtained PCR products of the predicted size from all samples, and we confirmed the presence of AEV via sequence analysis. The Japanese Society of Veterinary Science 2018-11-21 2019-01 /pmc/articles/PMC6361656/ /pubmed/30464075 http://dx.doi.org/10.1292/jvms.18-0550 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Avian Pathology
GOTO, Yusuke
YAEGASHI, Gakuji
KUMAGAI, Yoshihiro
OGASAWARA, Fusae
GOTO, Makiko
MASE, Masaji
Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR
title Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR
title_full Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR
title_fullStr Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR
title_full_unstemmed Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR
title_short Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR
title_sort detection of avian encephalomyelitis virus in chickens in japan using rt-pcr
topic Avian Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361656/
https://www.ncbi.nlm.nih.gov/pubmed/30464075
http://dx.doi.org/10.1292/jvms.18-0550
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