X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites

A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca....

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Detalles Bibliográficos
Autores principales: GAMO, Shiori, TOZAKI, Teruaki, KAKOI, Hironaga, HIROTA, Kei-ichi, NAKAMURA, Kotono, NISHII, Noriko, ALUMUNIA, Julio, TAKASU, Masaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Theriogenology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361659/
https://www.ncbi.nlm.nih.gov/pubmed/30473577
http://dx.doi.org/10.1292/jvms.18-0253
Descripción
Sumario:A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca.YM2, Eca.YA16, and the sex-determining region Y gene), and an X/Y marker (Amelogenin gene). This analysis demonstrated that the filly had not inherited an X chromosome from her sire. A karyotyping analysis confirmed that the filly was 63,XO. As it was suspected that the horse would be sterile, we avoided using the horse as a broodmare; the information should also serve to prevent unnecessary conflict between owners transferring and receiving the horse.

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