X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites

A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca....

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Autores principales: GAMO, Shiori, TOZAKI, Teruaki, KAKOI, Hironaga, HIROTA, Kei-ichi, NAKAMURA, Kotono, NISHII, Noriko, ALUMUNIA, Julio, TAKASU, Masaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Theriogenology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361659/
https://www.ncbi.nlm.nih.gov/pubmed/30473577
http://dx.doi.org/10.1292/jvms.18-0253
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author GAMO, Shiori
TOZAKI, Teruaki
KAKOI, Hironaga
HIROTA, Kei-ichi
NAKAMURA, Kotono
NISHII, Noriko
ALUMUNIA, Julio
TAKASU, Masaki
author_facet GAMO, Shiori
TOZAKI, Teruaki
KAKOI, Hironaga
HIROTA, Kei-ichi
NAKAMURA, Kotono
NISHII, Noriko
ALUMUNIA, Julio
TAKASU, Masaki
author_sort GAMO, Shiori
collection PubMed
description A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca.YM2, Eca.YA16, and the sex-determining region Y gene), and an X/Y marker (Amelogenin gene). This analysis demonstrated that the filly had not inherited an X chromosome from her sire. A karyotyping analysis confirmed that the filly was 63,XO. As it was suspected that the horse would be sterile, we avoided using the horse as a broodmare; the information should also serve to prevent unnecessary conflict between owners transferring and receiving the horse.
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spelling pubmed-63616592019-02-08 X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites GAMO, Shiori TOZAKI, Teruaki KAKOI, Hironaga HIROTA, Kei-ichi NAKAMURA, Kotono NISHII, Noriko ALUMUNIA, Julio TAKASU, Masaki J Vet Med Sci Theriogenology A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca.YM2, Eca.YA16, and the sex-determining region Y gene), and an X/Y marker (Amelogenin gene). This analysis demonstrated that the filly had not inherited an X chromosome from her sire. A karyotyping analysis confirmed that the filly was 63,XO. As it was suspected that the horse would be sterile, we avoided using the horse as a broodmare; the information should also serve to prevent unnecessary conflict between owners transferring and receiving the horse. The Japanese Society of Veterinary Science 2018-11-26 2019-01 /pmc/articles/PMC6361659/ /pubmed/30473577 http://dx.doi.org/10.1292/jvms.18-0253 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Theriogenology
GAMO, Shiori
TOZAKI, Teruaki
KAKOI, Hironaga
HIROTA, Kei-ichi
NAKAMURA, Kotono
NISHII, Noriko
ALUMUNIA, Julio
TAKASU, Masaki
X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites
title X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites
title_full X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites
title_fullStr X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites
title_full_unstemmed X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites
title_short X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites
title_sort x monosomy in the endangered kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites
topic Theriogenology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361659/
https://www.ncbi.nlm.nih.gov/pubmed/30473577
http://dx.doi.org/10.1292/jvms.18-0253
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