Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells

It has been reported that hypomagnetic fields (HMFs) have a negative influence on mammalian physiological functions. We previously reported that HMFs were detrimental to cell fate changes during reprogramming into pluripotency. These studies led us to investigate whether HMFs affect cell fate determ...

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Autores principales: Baek, Soonbong, Choi, Hwan, Park, Hanseul, Cho, Byunguk, Kim, Siyoung, Kim, Jongpil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361932/
https://www.ncbi.nlm.nih.gov/pubmed/30718529
http://dx.doi.org/10.1038/s41598-018-37372-2
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author Baek, Soonbong
Choi, Hwan
Park, Hanseul
Cho, Byunguk
Kim, Siyoung
Kim, Jongpil
author_facet Baek, Soonbong
Choi, Hwan
Park, Hanseul
Cho, Byunguk
Kim, Siyoung
Kim, Jongpil
author_sort Baek, Soonbong
collection PubMed
description It has been reported that hypomagnetic fields (HMFs) have a negative influence on mammalian physiological functions. We previously reported that HMFs were detrimental to cell fate changes during reprogramming into pluripotency. These studies led us to investigate whether HMFs affect cell fate determination during direct differentiation. Here, we found that an HMF environment attenuates differentiation capacity and is detrimental to cell fate changes during the in vitro differentiation of embryonic stem cells (ESCs). Moreover, HMF conditions cause abnormal DNA methylation through the dysregulation of DNA methyltransferase3b (Dnmt3b) expression, eventually resulting in incomplete DNA methylation during differentiation. Taken together, these results suggest that an appropriate electromagnetic field (EMF) environment may be essential for favorable epigenetic remodeling during cell fate determination via differentiation.
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spelling pubmed-63619322019-02-06 Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells Baek, Soonbong Choi, Hwan Park, Hanseul Cho, Byunguk Kim, Siyoung Kim, Jongpil Sci Rep Article It has been reported that hypomagnetic fields (HMFs) have a negative influence on mammalian physiological functions. We previously reported that HMFs were detrimental to cell fate changes during reprogramming into pluripotency. These studies led us to investigate whether HMFs affect cell fate determination during direct differentiation. Here, we found that an HMF environment attenuates differentiation capacity and is detrimental to cell fate changes during the in vitro differentiation of embryonic stem cells (ESCs). Moreover, HMF conditions cause abnormal DNA methylation through the dysregulation of DNA methyltransferase3b (Dnmt3b) expression, eventually resulting in incomplete DNA methylation during differentiation. Taken together, these results suggest that an appropriate electromagnetic field (EMF) environment may be essential for favorable epigenetic remodeling during cell fate determination via differentiation. Nature Publishing Group UK 2019-02-04 /pmc/articles/PMC6361932/ /pubmed/30718529 http://dx.doi.org/10.1038/s41598-018-37372-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Baek, Soonbong
Choi, Hwan
Park, Hanseul
Cho, Byunguk
Kim, Siyoung
Kim, Jongpil
Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells
title Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells
title_full Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells
title_fullStr Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells
title_full_unstemmed Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells
title_short Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells
title_sort effects of a hypomagnetic field on dna methylation during the differentiation of embryonic stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361932/
https://www.ncbi.nlm.nih.gov/pubmed/30718529
http://dx.doi.org/10.1038/s41598-018-37372-2
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