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Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

Although Cas9 nucleases are remarkably diverse in microorganisms, the range of genomic sequences targetable by a CRISPR/Cas9 system is restricted by the requirement of a short protospacer adjacent motif (PAM) at the target site. Here, we generate a group of chimeric Cas9 (cCas9) variants by replacin...

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Detalles Bibliográficos
Autores principales:	Ma, Dacheng, Xu, Zhimeng, Zhang, Zhaoyu, Chen, Xi, Zeng, Xiangzhi, Zhang, Yiyang, Deng, Tingyue, Ren, Mengfei, Sun, Zheng, Jiang, Rui, Xie, Zhen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361995/ https://www.ncbi.nlm.nih.gov/pubmed/30718489 http://dx.doi.org/10.1038/s41467-019-08395-8

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