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Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery

Our previous study introduced a barley protein microparticle for encapsulation of hydrophobic drug/nutraceutical, which could release nanoparticles upon gastric digestion and deliver encapsulated compound to a simulated intestinal environment intact. This work focused on evaluating the potential of...

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Autores principales: Liu, Guangyu, Zhou, Ying, Chen, Lingyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362262/
https://www.ncbi.nlm.nih.gov/pubmed/30766780
http://dx.doi.org/10.1016/j.apsb.2018.10.002
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author Liu, Guangyu
Zhou, Ying
Chen, Lingyun
author_facet Liu, Guangyu
Zhou, Ying
Chen, Lingyun
author_sort Liu, Guangyu
collection PubMed
description Our previous study introduced a barley protein microparticle for encapsulation of hydrophobic drug/nutraceutical, which could release nanoparticles upon gastric digestion and deliver encapsulated compound to a simulated intestinal environment intact. This work focused on evaluating the potential of liberated nanoparticles to improve the absorption of encapsulated compounds (e.g., β-carotene) using in vitro Caco-2 cell and ex vivo small intestine models. Nanoparticles obtained from gastric digestion of barley protein microparticles had a spherical shape and an average size of 351 nm. Nanoparticles showed low cytotoxicity in Caco-2 cells and their cellular uptake was dependent on time, concentration and temperature. In a Caco-2 cell monolayer model, significantly greater uptake and transport of β-carotene were observed when it was delivered by nanoparticles (15%), compared to free β-carotene suspension (2.6%). In an ex vivo rat jejunum model, nanoparticles showed the capacity to retain in small intestinal tissue. Approximately 2.24 and 6.04 μg nanoparticle were able to permeate through each cm(2) intestinal tissue and translocate to the serosal side after 60 and 90 min, respectively. Results from this study demonstrated the absorption improving effect of the barley protein nanoparticles and suggested their potential as vehicles for hydrophobic compounds.
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spelling pubmed-63622622019-02-14 Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery Liu, Guangyu Zhou, Ying Chen, Lingyun Acta Pharm Sin B Original article Our previous study introduced a barley protein microparticle for encapsulation of hydrophobic drug/nutraceutical, which could release nanoparticles upon gastric digestion and deliver encapsulated compound to a simulated intestinal environment intact. This work focused on evaluating the potential of liberated nanoparticles to improve the absorption of encapsulated compounds (e.g., β-carotene) using in vitro Caco-2 cell and ex vivo small intestine models. Nanoparticles obtained from gastric digestion of barley protein microparticles had a spherical shape and an average size of 351 nm. Nanoparticles showed low cytotoxicity in Caco-2 cells and their cellular uptake was dependent on time, concentration and temperature. In a Caco-2 cell monolayer model, significantly greater uptake and transport of β-carotene were observed when it was delivered by nanoparticles (15%), compared to free β-carotene suspension (2.6%). In an ex vivo rat jejunum model, nanoparticles showed the capacity to retain in small intestinal tissue. Approximately 2.24 and 6.04 μg nanoparticle were able to permeate through each cm(2) intestinal tissue and translocate to the serosal side after 60 and 90 min, respectively. Results from this study demonstrated the absorption improving effect of the barley protein nanoparticles and suggested their potential as vehicles for hydrophobic compounds. Elsevier 2019-01 2018-10-12 /pmc/articles/PMC6362262/ /pubmed/30766780 http://dx.doi.org/10.1016/j.apsb.2018.10.002 Text en © 2018 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Liu, Guangyu
Zhou, Ying
Chen, Lingyun
Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
title Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
title_full Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
title_fullStr Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
title_full_unstemmed Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
title_short Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
title_sort intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362262/
https://www.ncbi.nlm.nih.gov/pubmed/30766780
http://dx.doi.org/10.1016/j.apsb.2018.10.002
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