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Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery
Our previous study introduced a barley protein microparticle for encapsulation of hydrophobic drug/nutraceutical, which could release nanoparticles upon gastric digestion and deliver encapsulated compound to a simulated intestinal environment intact. This work focused on evaluating the potential of...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362262/ https://www.ncbi.nlm.nih.gov/pubmed/30766780 http://dx.doi.org/10.1016/j.apsb.2018.10.002 |
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author | Liu, Guangyu Zhou, Ying Chen, Lingyun |
author_facet | Liu, Guangyu Zhou, Ying Chen, Lingyun |
author_sort | Liu, Guangyu |
collection | PubMed |
description | Our previous study introduced a barley protein microparticle for encapsulation of hydrophobic drug/nutraceutical, which could release nanoparticles upon gastric digestion and deliver encapsulated compound to a simulated intestinal environment intact. This work focused on evaluating the potential of liberated nanoparticles to improve the absorption of encapsulated compounds (e.g., β-carotene) using in vitro Caco-2 cell and ex vivo small intestine models. Nanoparticles obtained from gastric digestion of barley protein microparticles had a spherical shape and an average size of 351 nm. Nanoparticles showed low cytotoxicity in Caco-2 cells and their cellular uptake was dependent on time, concentration and temperature. In a Caco-2 cell monolayer model, significantly greater uptake and transport of β-carotene were observed when it was delivered by nanoparticles (15%), compared to free β-carotene suspension (2.6%). In an ex vivo rat jejunum model, nanoparticles showed the capacity to retain in small intestinal tissue. Approximately 2.24 and 6.04 μg nanoparticle were able to permeate through each cm(2) intestinal tissue and translocate to the serosal side after 60 and 90 min, respectively. Results from this study demonstrated the absorption improving effect of the barley protein nanoparticles and suggested their potential as vehicles for hydrophobic compounds. |
format | Online Article Text |
id | pubmed-6362262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-63622622019-02-14 Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery Liu, Guangyu Zhou, Ying Chen, Lingyun Acta Pharm Sin B Original article Our previous study introduced a barley protein microparticle for encapsulation of hydrophobic drug/nutraceutical, which could release nanoparticles upon gastric digestion and deliver encapsulated compound to a simulated intestinal environment intact. This work focused on evaluating the potential of liberated nanoparticles to improve the absorption of encapsulated compounds (e.g., β-carotene) using in vitro Caco-2 cell and ex vivo small intestine models. Nanoparticles obtained from gastric digestion of barley protein microparticles had a spherical shape and an average size of 351 nm. Nanoparticles showed low cytotoxicity in Caco-2 cells and their cellular uptake was dependent on time, concentration and temperature. In a Caco-2 cell monolayer model, significantly greater uptake and transport of β-carotene were observed when it was delivered by nanoparticles (15%), compared to free β-carotene suspension (2.6%). In an ex vivo rat jejunum model, nanoparticles showed the capacity to retain in small intestinal tissue. Approximately 2.24 and 6.04 μg nanoparticle were able to permeate through each cm(2) intestinal tissue and translocate to the serosal side after 60 and 90 min, respectively. Results from this study demonstrated the absorption improving effect of the barley protein nanoparticles and suggested their potential as vehicles for hydrophobic compounds. Elsevier 2019-01 2018-10-12 /pmc/articles/PMC6362262/ /pubmed/30766780 http://dx.doi.org/10.1016/j.apsb.2018.10.002 Text en © 2018 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original article Liu, Guangyu Zhou, Ying Chen, Lingyun Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery |
title | Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery |
title_full | Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery |
title_fullStr | Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery |
title_full_unstemmed | Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery |
title_short | Intestinal uptake of barley protein-based nanoparticles for β-carotene delivery |
title_sort | intestinal uptake of barley protein-based nanoparticles for β-carotene delivery |
topic | Original article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362262/ https://www.ncbi.nlm.nih.gov/pubmed/30766780 http://dx.doi.org/10.1016/j.apsb.2018.10.002 |
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