Structural biology workflow for the expression and characterization of functional human sodium glucose transporter type 1 in Pichia pastoris

Heterologous expression of human membrane proteins is a challenge in structural biology towards drug discovery. Here we report a complete expression and purification process of a functional human sodium/D-glucose co-transporter 1 (hSGLT1) in Pichia pastoris as representative example of a useful stra...

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Detalles Bibliográficos
Autores principales: Suades, Albert, Alcaraz, Antonio, Cruz, Esteban, Álvarez-Marimon, Elena, Whitelegge, Julian P., Manyosa, Joan, Cladera, Josep, Perálvarez-Marín, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362292/
https://www.ncbi.nlm.nih.gov/pubmed/30718602
http://dx.doi.org/10.1038/s41598-018-37445-2

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