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NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity
Recognition of microbe-associated molecular patterns (MAMPs) derived from invading pathogens by plant pattern recognition receptors (PRRs) initiates a subset of defense responses known as pattern-triggered immunity (PTI). Transcription factors (TFs) orchestrate the onset of PTI through complex signa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363091/ https://www.ncbi.nlm.nih.gov/pubmed/30462256 http://dx.doi.org/10.1093/jxb/ery414 |
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author | Huang, Pin-Yao Zhang, Jingsong Jiang, Beier Chan, Ching Yu, Jhong-He Lu, Yu-Pin Chung, KwiMi Zimmerli, Laurent |
author_facet | Huang, Pin-Yao Zhang, Jingsong Jiang, Beier Chan, Ching Yu, Jhong-He Lu, Yu-Pin Chung, KwiMi Zimmerli, Laurent |
author_sort | Huang, Pin-Yao |
collection | PubMed |
description | Recognition of microbe-associated molecular patterns (MAMPs) derived from invading pathogens by plant pattern recognition receptors (PRRs) initiates a subset of defense responses known as pattern-triggered immunity (PTI). Transcription factors (TFs) orchestrate the onset of PTI through complex signaling networks. Here, we characterized the function of ERF19, a member of the Arabidopsis thaliana ethylene response factor (ERF) family. ERF19 was found to act as a negative regulator of PTI against Botrytis cinerea and Pseudomonas syringae. Notably, overexpression of ERF19 increased plant susceptibility to these pathogens and repressed MAMP-induced PTI outputs. In contrast, expression of the chimeric dominant repressor ERF19–SRDX boosted PTI activation, conferred increased resistance to the fungus B. cinerea, and enhanced elf18-triggered immunity against bacteria. Consistent with a negative role for ERF19 in PTI, MAMP-mediated growth inhibition was weakened or augmented in lines overexpressing ERF19 or expressing ERF19–SRDX, respectively. Using biochemical and genetic approaches, we show that the transcriptional co-repressor Novel INteractor of JAZ (NINJA) associates with and represses the function of ERF19. Our work reveals ERF19 as a novel player in the mitigation of PTI, and highlights a potential role for NINJA in fine-tuning ERF19-mediated regulation of Arabidopsis innate immunity. |
format | Online Article Text |
id | pubmed-6363091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-63630912019-02-08 NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity Huang, Pin-Yao Zhang, Jingsong Jiang, Beier Chan, Ching Yu, Jhong-He Lu, Yu-Pin Chung, KwiMi Zimmerli, Laurent J Exp Bot Research Papers Recognition of microbe-associated molecular patterns (MAMPs) derived from invading pathogens by plant pattern recognition receptors (PRRs) initiates a subset of defense responses known as pattern-triggered immunity (PTI). Transcription factors (TFs) orchestrate the onset of PTI through complex signaling networks. Here, we characterized the function of ERF19, a member of the Arabidopsis thaliana ethylene response factor (ERF) family. ERF19 was found to act as a negative regulator of PTI against Botrytis cinerea and Pseudomonas syringae. Notably, overexpression of ERF19 increased plant susceptibility to these pathogens and repressed MAMP-induced PTI outputs. In contrast, expression of the chimeric dominant repressor ERF19–SRDX boosted PTI activation, conferred increased resistance to the fungus B. cinerea, and enhanced elf18-triggered immunity against bacteria. Consistent with a negative role for ERF19 in PTI, MAMP-mediated growth inhibition was weakened or augmented in lines overexpressing ERF19 or expressing ERF19–SRDX, respectively. Using biochemical and genetic approaches, we show that the transcriptional co-repressor Novel INteractor of JAZ (NINJA) associates with and represses the function of ERF19. Our work reveals ERF19 as a novel player in the mitigation of PTI, and highlights a potential role for NINJA in fine-tuning ERF19-mediated regulation of Arabidopsis innate immunity. Oxford University Press 2019-01-30 2018-11-21 /pmc/articles/PMC6363091/ /pubmed/30462256 http://dx.doi.org/10.1093/jxb/ery414 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Huang, Pin-Yao Zhang, Jingsong Jiang, Beier Chan, Ching Yu, Jhong-He Lu, Yu-Pin Chung, KwiMi Zimmerli, Laurent NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity |
title | NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity |
title_full | NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity |
title_fullStr | NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity |
title_full_unstemmed | NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity |
title_short | NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity |
title_sort | ninja-associated erf19 negatively regulates arabidopsis pattern-triggered immunity |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363091/ https://www.ncbi.nlm.nih.gov/pubmed/30462256 http://dx.doi.org/10.1093/jxb/ery414 |
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