Cargando…
Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines
Wild relatives of wheat, such as Aegilops spp. are potential sources of genes conferring tolerance to drought stress. As drought stress affects seed composition, the main goal of the present study was to determine the effects of drought stress on the content and composition of the grain storage prot...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363227/ https://www.ncbi.nlm.nih.gov/pubmed/30721262 http://dx.doi.org/10.1371/journal.pone.0211892 |
_version_ | 1783393068348604416 |
---|---|
author | Rakszegi, Marianna Darkó, Éva Lovegrove, Alison Molnár, István Láng, László Bedő, Zoltán Molnár-Láng, Márta Shewry, Peter |
author_facet | Rakszegi, Marianna Darkó, Éva Lovegrove, Alison Molnár, István Láng, László Bedő, Zoltán Molnár-Láng, Márta Shewry, Peter |
author_sort | Rakszegi, Marianna |
collection | PubMed |
description | Wild relatives of wheat, such as Aegilops spp. are potential sources of genes conferring tolerance to drought stress. As drought stress affects seed composition, the main goal of the present study was to determine the effects of drought stress on the content and composition of the grain storage protein (gliadin (Gli), glutenin (Glu), unextractable polymeric proteins (UPP%) and dietary fiber (arabinoxylan, β-glucan) components of hexaploid bread wheat (T. aestivum) lines containing added chromosomes from Ae. biuncialis or Ae. geniculata. Both Aegilops parents have higher contents of protein and β-glucan and higher proportions of water-soluble arabinoxylans (determined as pentosans) than wheat when grown under both well-watered and drought stress conditions. In general, drought stress resulted in increased contents of protein and total pentosans in the addition lines, while the β-glucan content decreased in many of the addition lines. The differences found between the wheat/Aegilops addition lines and wheat parents under well-watered conditions were also manifested under drought stress conditions: Namely, elevated β-glucan content was found in addition lines containing chromosomes 5U(g), 7U(g) and 7M(b), while chromosomes 1U(b) and 1M(g) affected the proportion of polymeric proteins (determined as Glu/Gli and UPP%, respectively) under both well-watered and drought stress conditions. Furthermore, the addition of chromosome 6M(g) decreased the WE-pentosan content under both conditions. The grain composition of the Aegilops accessions was more stable under drought stress than that of wheat, and wheat lines with the added Aegilops chromosomes 2M(g) and 5M(g) also had more stable grain protein and pentosan contents. The negative effects of drought stress on both the physical and compositional properties of wheat were also reduced by the addition of these. These results suggest that the stability of the grain composition could be improved under drought stress conditions by the intraspecific hybridization of wheat with its wild relatives. |
format | Online Article Text |
id | pubmed-6363227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63632272019-02-15 Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines Rakszegi, Marianna Darkó, Éva Lovegrove, Alison Molnár, István Láng, László Bedő, Zoltán Molnár-Láng, Márta Shewry, Peter PLoS One Research Article Wild relatives of wheat, such as Aegilops spp. are potential sources of genes conferring tolerance to drought stress. As drought stress affects seed composition, the main goal of the present study was to determine the effects of drought stress on the content and composition of the grain storage protein (gliadin (Gli), glutenin (Glu), unextractable polymeric proteins (UPP%) and dietary fiber (arabinoxylan, β-glucan) components of hexaploid bread wheat (T. aestivum) lines containing added chromosomes from Ae. biuncialis or Ae. geniculata. Both Aegilops parents have higher contents of protein and β-glucan and higher proportions of water-soluble arabinoxylans (determined as pentosans) than wheat when grown under both well-watered and drought stress conditions. In general, drought stress resulted in increased contents of protein and total pentosans in the addition lines, while the β-glucan content decreased in many of the addition lines. The differences found between the wheat/Aegilops addition lines and wheat parents under well-watered conditions were also manifested under drought stress conditions: Namely, elevated β-glucan content was found in addition lines containing chromosomes 5U(g), 7U(g) and 7M(b), while chromosomes 1U(b) and 1M(g) affected the proportion of polymeric proteins (determined as Glu/Gli and UPP%, respectively) under both well-watered and drought stress conditions. Furthermore, the addition of chromosome 6M(g) decreased the WE-pentosan content under both conditions. The grain composition of the Aegilops accessions was more stable under drought stress than that of wheat, and wheat lines with the added Aegilops chromosomes 2M(g) and 5M(g) also had more stable grain protein and pentosan contents. The negative effects of drought stress on both the physical and compositional properties of wheat were also reduced by the addition of these. These results suggest that the stability of the grain composition could be improved under drought stress conditions by the intraspecific hybridization of wheat with its wild relatives. Public Library of Science 2019-02-05 /pmc/articles/PMC6363227/ /pubmed/30721262 http://dx.doi.org/10.1371/journal.pone.0211892 Text en © 2019 Rakszegi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rakszegi, Marianna Darkó, Éva Lovegrove, Alison Molnár, István Láng, László Bedő, Zoltán Molnár-Láng, Márta Shewry, Peter Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines |
title | Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines |
title_full | Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines |
title_fullStr | Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines |
title_full_unstemmed | Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines |
title_short | Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines |
title_sort | drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/aegilops addition lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363227/ https://www.ncbi.nlm.nih.gov/pubmed/30721262 http://dx.doi.org/10.1371/journal.pone.0211892 |
work_keys_str_mv | AT rakszegimarianna droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines AT darkoeva droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines AT lovegrovealison droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines AT molnaristvan droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines AT langlaszlo droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines AT bedozoltan droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines AT molnarlangmarta droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines AT shewrypeter droughtstressaffectstheproteinanddietaryfibercontentofwholemealwheatflourinwheataegilopsadditionlines |