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Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory

Leptospirosis is the most emerging zoonotic disease of epidemic potential caused by pathogenic species of Leptospira. The bacterium invades the host system and causes the disease by interacting with the host proteins. Analyzing these pathogen-host protein interactions (PHPIs) may provide deeper insi...

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Autores principales: Kumar, Swapnil, Lata, Kumari Snehkant, Sharma, Priyanka, Bhairappanavar, Shivarudrappa B., Soni, Subhash, Das, Jayashankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363727/
https://www.ncbi.nlm.nih.gov/pubmed/30723266
http://dx.doi.org/10.1038/s41598-018-38329-1
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author Kumar, Swapnil
Lata, Kumari Snehkant
Sharma, Priyanka
Bhairappanavar, Shivarudrappa B.
Soni, Subhash
Das, Jayashankar
author_facet Kumar, Swapnil
Lata, Kumari Snehkant
Sharma, Priyanka
Bhairappanavar, Shivarudrappa B.
Soni, Subhash
Das, Jayashankar
author_sort Kumar, Swapnil
collection PubMed
description Leptospirosis is the most emerging zoonotic disease of epidemic potential caused by pathogenic species of Leptospira. The bacterium invades the host system and causes the disease by interacting with the host proteins. Analyzing these pathogen-host protein interactions (PHPIs) may provide deeper insight into the disease pathogenesis. For this analysis, inter-species as well as intra-species protein interactions networks of Leptospira interrogans and human were constructed and investigated. The topological analyses of these networks showed lesser connectivity in inter-species network than intra-species, indicating the perturbed nature of the inter-species network. Hence, it can be one of the reasons behind the disease development. A total of 35 out of 586 PHPIs were identified as key interactions based on their sub-cellular localization. Two outer membrane proteins (GpsA and MetXA) and two periplasmic proteins (Flab and GlyA) participating in PHPIs were found conserved in all pathogenic, intermediate and saprophytic spp. of Leptospira. Furthermore, the bacterial membrane proteins involved in PHPIs were found playing major roles in disruption of the immune systems and metabolic processes within host and thereby causing infectious disease. Thus, the present results signify that the membrane proteins participating in such interactions hold potential to serve as effective immunotherapeutic candidates for vaccine development.
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spelling pubmed-63637272019-02-07 Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory Kumar, Swapnil Lata, Kumari Snehkant Sharma, Priyanka Bhairappanavar, Shivarudrappa B. Soni, Subhash Das, Jayashankar Sci Rep Article Leptospirosis is the most emerging zoonotic disease of epidemic potential caused by pathogenic species of Leptospira. The bacterium invades the host system and causes the disease by interacting with the host proteins. Analyzing these pathogen-host protein interactions (PHPIs) may provide deeper insight into the disease pathogenesis. For this analysis, inter-species as well as intra-species protein interactions networks of Leptospira interrogans and human were constructed and investigated. The topological analyses of these networks showed lesser connectivity in inter-species network than intra-species, indicating the perturbed nature of the inter-species network. Hence, it can be one of the reasons behind the disease development. A total of 35 out of 586 PHPIs were identified as key interactions based on their sub-cellular localization. Two outer membrane proteins (GpsA and MetXA) and two periplasmic proteins (Flab and GlyA) participating in PHPIs were found conserved in all pathogenic, intermediate and saprophytic spp. of Leptospira. Furthermore, the bacterial membrane proteins involved in PHPIs were found playing major roles in disruption of the immune systems and metabolic processes within host and thereby causing infectious disease. Thus, the present results signify that the membrane proteins participating in such interactions hold potential to serve as effective immunotherapeutic candidates for vaccine development. Nature Publishing Group UK 2019-02-05 /pmc/articles/PMC6363727/ /pubmed/30723266 http://dx.doi.org/10.1038/s41598-018-38329-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kumar, Swapnil
Lata, Kumari Snehkant
Sharma, Priyanka
Bhairappanavar, Shivarudrappa B.
Soni, Subhash
Das, Jayashankar
Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory
title Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory
title_full Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory
title_fullStr Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory
title_full_unstemmed Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory
title_short Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory
title_sort inferring pathogen-host interactions between leptospira interrogans and homo sapiens using network theory
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363727/
https://www.ncbi.nlm.nih.gov/pubmed/30723266
http://dx.doi.org/10.1038/s41598-018-38329-1
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