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Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa

Cellulose synthase-like D (CSLD) family was characterized for their expression and functions in Populus trichocarpa. Ten members, PtrCslD1-10, were identified in the P. trichocarpa genome, and they belong to 4 clades by phylogenetic tree analysis. qRT-PCR and promoter:GUS assays in Arabidopsis and P...

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Autores principales: Peng, Xiaopeng, Pang, Hongying, Abbas, Manzar, Yan, Xiaojing, Dai, Xinren, Li, Yun, Li, Quanzi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363781/
https://www.ncbi.nlm.nih.gov/pubmed/30723218
http://dx.doi.org/10.1038/s41598-018-36529-3
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author Peng, Xiaopeng
Pang, Hongying
Abbas, Manzar
Yan, Xiaojing
Dai, Xinren
Li, Yun
Li, Quanzi
author_facet Peng, Xiaopeng
Pang, Hongying
Abbas, Manzar
Yan, Xiaojing
Dai, Xinren
Li, Yun
Li, Quanzi
author_sort Peng, Xiaopeng
collection PubMed
description Cellulose synthase-like D (CSLD) family was characterized for their expression and functions in Populus trichocarpa. Ten members, PtrCslD1-10, were identified in the P. trichocarpa genome, and they belong to 4 clades by phylogenetic tree analysis. qRT-PCR and promoter:GUS assays in Arabidopsis and P. trichocarpa displayed divergent expression patterns of these 10 PtrCSLD genes in root hairs, root tips, leaves, vascular tissues, xylem and flowers. Among PtrCslD2, PtrCslD4, PtrCslD5, PtrCslD6, and PtrCslD8 that all exhibited expression in root hairs, only PtrCslD5 could restore the root hairless phenotype of the atcsld3 mutant, demonstrating that PtrCslD5 is the functional ortholog of AtCslD3 for root hair formation. Our results suggest more possible functions for other PtrCslD genes in poplar.
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spelling pubmed-63637812019-02-07 Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa Peng, Xiaopeng Pang, Hongying Abbas, Manzar Yan, Xiaojing Dai, Xinren Li, Yun Li, Quanzi Sci Rep Article Cellulose synthase-like D (CSLD) family was characterized for their expression and functions in Populus trichocarpa. Ten members, PtrCslD1-10, were identified in the P. trichocarpa genome, and they belong to 4 clades by phylogenetic tree analysis. qRT-PCR and promoter:GUS assays in Arabidopsis and P. trichocarpa displayed divergent expression patterns of these 10 PtrCSLD genes in root hairs, root tips, leaves, vascular tissues, xylem and flowers. Among PtrCslD2, PtrCslD4, PtrCslD5, PtrCslD6, and PtrCslD8 that all exhibited expression in root hairs, only PtrCslD5 could restore the root hairless phenotype of the atcsld3 mutant, demonstrating that PtrCslD5 is the functional ortholog of AtCslD3 for root hair formation. Our results suggest more possible functions for other PtrCslD genes in poplar. Nature Publishing Group UK 2019-02-05 /pmc/articles/PMC6363781/ /pubmed/30723218 http://dx.doi.org/10.1038/s41598-018-36529-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Peng, Xiaopeng
Pang, Hongying
Abbas, Manzar
Yan, Xiaojing
Dai, Xinren
Li, Yun
Li, Quanzi
Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa
title Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa
title_full Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa
title_fullStr Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa
title_full_unstemmed Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa
title_short Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa
title_sort characterization of cellulose synthase-like d (csld) family revealed the involvement of ptrcsld5 in root hair formation in populus trichocarpa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363781/
https://www.ncbi.nlm.nih.gov/pubmed/30723218
http://dx.doi.org/10.1038/s41598-018-36529-3
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