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MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1

Cardiovascular ischemic disease refers to a large class of conditions that are harmful to human health. A number of previous studies have demonstrated that microRNAs (miRs) have notable roles in regulating cardiac injury. miR-144 is influential in the differentiation, growth, and metastatic processe...

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Detalles Bibliográficos
Autores principales: E, Lusha, Jiang, Hong, Lu, Zhibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364149/
https://www.ncbi.nlm.nih.gov/pubmed/30783480
http://dx.doi.org/10.3892/etm.2019.7161
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author E, Lusha
Jiang, Hong
Lu, Zhibing
author_facet E, Lusha
Jiang, Hong
Lu, Zhibing
author_sort E, Lusha
collection PubMed
description Cardiovascular ischemic disease refers to a large class of conditions that are harmful to human health. A number of previous studies have demonstrated that microRNAs (miRs) have notable roles in regulating cardiac injury. miR-144 is influential in the differentiation, growth, and metastatic processes of cells; however, the impact of miR-144 in cardiac ischemia/reperfusion (I/R) injury has not been thoroughly elucidated to date. In the present study, reverse transcription quantitative polymerase chain reaction was used to evaluate RNA expression. In addition, TTC staining was performed to detect the infarct area of the ischemic myocardia and a terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay was utilized to detect the apoptosis of the myocardia. It was observed that miR-144 expression is downregulated in an I/R model in rats and that overexpression of miR-144 significantly reduced myocardial ischemic injury and apoptosis. Consistent with this result, similar findings were demonstrated in H9c2 cells subjected to hypoxia/reoxygenation. Bioinformatic analysis using MiRanda and TargetScan, and luciferase assays confirmed that forkhead box protein O1was the target of miR-144. These findings suggest that miR-144 may be exploited as a novel molecular marker or therapeutic target for myocardial I/R injury.
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spelling pubmed-63641492019-02-19 MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1 E, Lusha Jiang, Hong Lu, Zhibing Exp Ther Med Articles Cardiovascular ischemic disease refers to a large class of conditions that are harmful to human health. A number of previous studies have demonstrated that microRNAs (miRs) have notable roles in regulating cardiac injury. miR-144 is influential in the differentiation, growth, and metastatic processes of cells; however, the impact of miR-144 in cardiac ischemia/reperfusion (I/R) injury has not been thoroughly elucidated to date. In the present study, reverse transcription quantitative polymerase chain reaction was used to evaluate RNA expression. In addition, TTC staining was performed to detect the infarct area of the ischemic myocardia and a terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay was utilized to detect the apoptosis of the myocardia. It was observed that miR-144 expression is downregulated in an I/R model in rats and that overexpression of miR-144 significantly reduced myocardial ischemic injury and apoptosis. Consistent with this result, similar findings were demonstrated in H9c2 cells subjected to hypoxia/reoxygenation. Bioinformatic analysis using MiRanda and TargetScan, and luciferase assays confirmed that forkhead box protein O1was the target of miR-144. These findings suggest that miR-144 may be exploited as a novel molecular marker or therapeutic target for myocardial I/R injury. D.A. Spandidos 2019-03 2019-01-09 /pmc/articles/PMC6364149/ /pubmed/30783480 http://dx.doi.org/10.3892/etm.2019.7161 Text en Copyright: © E et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
E, Lusha
Jiang, Hong
Lu, Zhibing
MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1
title MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1
title_full MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1
title_fullStr MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1
title_full_unstemmed MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1
title_short MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1
title_sort microrna-144 attenuates cardiac ischemia/reperfusion injury by targeting foxo1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364149/
https://www.ncbi.nlm.nih.gov/pubmed/30783480
http://dx.doi.org/10.3892/etm.2019.7161
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