Cargando…
Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells
INTRODUCTION: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA. MATERIAL AND METHODS: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at conc...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364151/ https://www.ncbi.nlm.nih.gov/pubmed/30729212 http://dx.doi.org/10.2478/jvetres-2018-0066 |
_version_ | 1783393210912997376 |
---|---|
author | Terpiłowska, Sylwia Siwicka-Gieroba, Dorota Siwicki, Andrzej Krzysztof |
author_facet | Terpiłowska, Sylwia Siwicka-Gieroba, Dorota Siwicki, Andrzej Krzysztof |
author_sort | Terpiłowska, Sylwia |
collection | PubMed |
description | INTRODUCTION: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA. MATERIAL AND METHODS: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 μM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests. RESULTS: A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays. CONCLUSION: Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed. |
format | Online Article Text |
id | pubmed-6364151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-63641512019-02-06 Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells Terpiłowska, Sylwia Siwicka-Gieroba, Dorota Siwicki, Andrzej Krzysztof J Vet Res Research Article INTRODUCTION: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA. MATERIAL AND METHODS: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 μM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests. RESULTS: A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays. CONCLUSION: Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed. Sciendo 2018-12-31 /pmc/articles/PMC6364151/ /pubmed/30729212 http://dx.doi.org/10.2478/jvetres-2018-0066 Text en © 2018 S. Terpiłowska et al. published by Sciendo http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Research Article Terpiłowska, Sylwia Siwicka-Gieroba, Dorota Siwicki, Andrzej Krzysztof Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells |
title | Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells |
title_full | Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells |
title_fullStr | Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells |
title_full_unstemmed | Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells |
title_short | Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells |
title_sort | cytotoxicity of iron (iii), molybdenum (iii), and their mixtures in balb/3t3 and hepg2 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364151/ https://www.ncbi.nlm.nih.gov/pubmed/30729212 http://dx.doi.org/10.2478/jvetres-2018-0066 |
work_keys_str_mv | AT terpiłowskasylwia cytotoxicityofironiiimolybdenumiiiandtheirmixturesinbalb3t3andhepg2cells AT siwickagierobadorota cytotoxicityofironiiimolybdenumiiiandtheirmixturesinbalb3t3andhepg2cells AT siwickiandrzejkrzysztof cytotoxicityofironiiimolybdenumiiiandtheirmixturesinbalb3t3andhepg2cells |