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Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells

INTRODUCTION: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA. MATERIAL AND METHODS: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at conc...

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Autores principales: Terpiłowska, Sylwia, Siwicka-Gieroba, Dorota, Siwicki, Andrzej Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364151/
https://www.ncbi.nlm.nih.gov/pubmed/30729212
http://dx.doi.org/10.2478/jvetres-2018-0066
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author Terpiłowska, Sylwia
Siwicka-Gieroba, Dorota
Siwicki, Andrzej Krzysztof
author_facet Terpiłowska, Sylwia
Siwicka-Gieroba, Dorota
Siwicki, Andrzej Krzysztof
author_sort Terpiłowska, Sylwia
collection PubMed
description INTRODUCTION: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA. MATERIAL AND METHODS: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 μM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests. RESULTS: A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays. CONCLUSION: Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed.
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spelling pubmed-63641512019-02-06 Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells Terpiłowska, Sylwia Siwicka-Gieroba, Dorota Siwicki, Andrzej Krzysztof J Vet Res Research Article INTRODUCTION: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA. MATERIAL AND METHODS: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 μM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests. RESULTS: A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays. CONCLUSION: Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed. Sciendo 2018-12-31 /pmc/articles/PMC6364151/ /pubmed/30729212 http://dx.doi.org/10.2478/jvetres-2018-0066 Text en © 2018 S. Terpiłowska et al. published by Sciendo http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
spellingShingle Research Article
Terpiłowska, Sylwia
Siwicka-Gieroba, Dorota
Siwicki, Andrzej Krzysztof
Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells
title Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells
title_full Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells
title_fullStr Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells
title_full_unstemmed Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells
title_short Cytotoxicity of Iron (III), Molybdenum (III), and their Mixtures in BALB/3T3 and HepG2 Cells
title_sort cytotoxicity of iron (iii), molybdenum (iii), and their mixtures in balb/3t3 and hepg2 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364151/
https://www.ncbi.nlm.nih.gov/pubmed/30729212
http://dx.doi.org/10.2478/jvetres-2018-0066
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