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Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle
INTRODUCTION: Coxiella (C.) burnetii, the aetiological agent of Q fever, is able to modulate the macrophage/T-lymphocyte axis in an infected organism and impair synthesis of monokines and lymphokines. MATERIAL AND METHODS: The purpose of this research was to determine the levels of the cytokines tha...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364170/ https://www.ncbi.nlm.nih.gov/pubmed/30729204 http://dx.doi.org/10.2478/jvetres-2018-0076 |
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author | Małaczewska, Joanna Kaczorek-Łukowska, Edyta Szymańska-Czerwińska, Monika Rękawek, Wojciech Wójcik, Roman Niemczuk, Krzysztof Siwicki, Andrzej Krzysztof |
author_facet | Małaczewska, Joanna Kaczorek-Łukowska, Edyta Szymańska-Czerwińska, Monika Rękawek, Wojciech Wójcik, Roman Niemczuk, Krzysztof Siwicki, Andrzej Krzysztof |
author_sort | Małaczewska, Joanna |
collection | PubMed |
description | INTRODUCTION: Coxiella (C.) burnetii, the aetiological agent of Q fever, is able to modulate the macrophage/T-lymphocyte axis in an infected organism and impair synthesis of monokines and lymphokines. MATERIAL AND METHODS: The purpose of this research was to determine the levels of the cytokines that play a key role in the response to C. burnetii antigens (IL-1β, IL-2, IL-6, IL-10, IFN-γ and TNF-α) in the serum of animals originating from an infected herd prior to vaccination (day 0) and at 1, 7, and 21 days afterwards. RESULTS: The vaccination of animals did not affect the production of IL-6, IL-1β, or IL-2. The serum levels of these cytokines were too low to measure in most of the samples. The initial levels of TNFα, IFNγ, and IL-10 were higher in seropositive than in seronegative animals, although significant differences between seropositive shedders and seropositive nonshedders appeared only in the levels of IFNγ and IL-10. Additionally, the course of the post-vaccination response concerning these two cytokines was different among seronegative nonshedders, seropositive nonshedders, and seropositive shedders. CONCLUSION: It seems that analysis of the IFNγ and IL-10 concentrations in animal blood serum may have some practical value in an assessment of the health status of seropositive animals and post-vaccination response. |
format | Online Article Text |
id | pubmed-6364170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-63641702019-02-06 Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle Małaczewska, Joanna Kaczorek-Łukowska, Edyta Szymańska-Czerwińska, Monika Rękawek, Wojciech Wójcik, Roman Niemczuk, Krzysztof Siwicki, Andrzej Krzysztof J Vet Res Research Article INTRODUCTION: Coxiella (C.) burnetii, the aetiological agent of Q fever, is able to modulate the macrophage/T-lymphocyte axis in an infected organism and impair synthesis of monokines and lymphokines. MATERIAL AND METHODS: The purpose of this research was to determine the levels of the cytokines that play a key role in the response to C. burnetii antigens (IL-1β, IL-2, IL-6, IL-10, IFN-γ and TNF-α) in the serum of animals originating from an infected herd prior to vaccination (day 0) and at 1, 7, and 21 days afterwards. RESULTS: The vaccination of animals did not affect the production of IL-6, IL-1β, or IL-2. The serum levels of these cytokines were too low to measure in most of the samples. The initial levels of TNFα, IFNγ, and IL-10 were higher in seropositive than in seronegative animals, although significant differences between seropositive shedders and seropositive nonshedders appeared only in the levels of IFNγ and IL-10. Additionally, the course of the post-vaccination response concerning these two cytokines was different among seronegative nonshedders, seropositive nonshedders, and seropositive shedders. CONCLUSION: It seems that analysis of the IFNγ and IL-10 concentrations in animal blood serum may have some practical value in an assessment of the health status of seropositive animals and post-vaccination response. Sciendo 2018-12-31 /pmc/articles/PMC6364170/ /pubmed/30729204 http://dx.doi.org/10.2478/jvetres-2018-0076 Text en © 2018 J. Małaczewska et al. published by Sciendo http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Research Article Małaczewska, Joanna Kaczorek-Łukowska, Edyta Szymańska-Czerwińska, Monika Rękawek, Wojciech Wójcik, Roman Niemczuk, Krzysztof Siwicki, Andrzej Krzysztof Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle |
title | Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle |
title_full | Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle |
title_fullStr | Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle |
title_full_unstemmed | Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle |
title_short | Early Cytokine Response After Vaccination with Coxiella Burnetii Phase I in an Infected Herd of Dairy Cattle |
title_sort | early cytokine response after vaccination with coxiella burnetii phase i in an infected herd of dairy cattle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364170/ https://www.ncbi.nlm.nih.gov/pubmed/30729204 http://dx.doi.org/10.2478/jvetres-2018-0076 |
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