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Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq
BACKGROUND: Citrus plants are commercially propagated by grafting, with the rootstock variety influencing a number of horticultural traits, including drought tolerance. Among the different rootstock varieties available for citrus propagation, ‘Rangpur’ lime is known to confer enhanced tolerance to d...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364419/ https://www.ncbi.nlm.nih.gov/pubmed/30727949 http://dx.doi.org/10.1186/s12864-019-5481-z |
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author | Gonçalves, Luana P. Boscariol Camargo, Raquel L. Takita, Marco Aurélio Machado, Marcos A. dos Soares Filho, Walter S. Costa, Marcio G. C. |
author_facet | Gonçalves, Luana P. Boscariol Camargo, Raquel L. Takita, Marco Aurélio Machado, Marcos A. dos Soares Filho, Walter S. Costa, Marcio G. C. |
author_sort | Gonçalves, Luana P. |
collection | PubMed |
description | BACKGROUND: Citrus plants are commercially propagated by grafting, with the rootstock variety influencing a number of horticultural traits, including drought tolerance. Among the different rootstock varieties available for citrus propagation, ‘Rangpur’ lime is known to confer enhanced tolerance to drought as compared to other citrus rootstocks. The objective of this study was to investigate the poorly understood molecular responses underlying the rootstock-induced drought tolerance in sweet orange. RESULTS: RNA-Seq transcriptome analysis was carried out in leaves of sweet orange grafted on ‘Rangpur’ lime subjected to control and drought-stress treatments, under greenhouse conditions, using the Illumina HiSeq platform. A total of 41,827 unique transcripts were identified, among which 1764 transcripts showed significant variation (P ≤ 0.001) between the treatments, with 1081 genes induced and 683 repressed by drought-stress treatment. The transcripts were distributed in 44 different categories of cellular component, molecular function and biological process. Several genes related to cell metabolism, including those involved in the metabolisms of cell wall, carbohydrates and antioxidants, light reactions, biotic and abiotic stress responses, as well as genes coding for transcription factors (TFs), protein kinases (PKs) and proteins involved in the abscisic acid (ABA) and ethylene signaling pathways, were differentially regulated by drought stress. RNA-Seq data were validated by quantitative real-time PCR (qPCR) analysis and comparative analysis of expression of the selected genes between sweet orange grafted on drought-tolerant and -sensitive rootstocks revealed new candidate genes for drought tolerance in citrus. CONCLUSIONS: In conclusion, our results showed that only a relatively small but functionally diverse fraction of the sweet orange transcriptome, with functions in metabolism, cellular responses and regulation, was differentially regulated by drought stress. The data suggest that the rootstock-induced drought tolerance in sweet orange includes the transcriptional activation of genes related to the cell wall, soluble carbohydrate and antioxidant metabolisms, biotic and abiotic stress responses, TFs, PKs and ABA signaling pathway, and the downregulation of genes involved in the starch metabolism, light reactions and ethylene signaling. Future efforts to elucidate their functional roles and explore their potential in the citrus genetic improvement should benefit from this data. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5481-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6364419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63644192019-02-15 Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq Gonçalves, Luana P. Boscariol Camargo, Raquel L. Takita, Marco Aurélio Machado, Marcos A. dos Soares Filho, Walter S. Costa, Marcio G. C. BMC Genomics Research Article BACKGROUND: Citrus plants are commercially propagated by grafting, with the rootstock variety influencing a number of horticultural traits, including drought tolerance. Among the different rootstock varieties available for citrus propagation, ‘Rangpur’ lime is known to confer enhanced tolerance to drought as compared to other citrus rootstocks. The objective of this study was to investigate the poorly understood molecular responses underlying the rootstock-induced drought tolerance in sweet orange. RESULTS: RNA-Seq transcriptome analysis was carried out in leaves of sweet orange grafted on ‘Rangpur’ lime subjected to control and drought-stress treatments, under greenhouse conditions, using the Illumina HiSeq platform. A total of 41,827 unique transcripts were identified, among which 1764 transcripts showed significant variation (P ≤ 0.001) between the treatments, with 1081 genes induced and 683 repressed by drought-stress treatment. The transcripts were distributed in 44 different categories of cellular component, molecular function and biological process. Several genes related to cell metabolism, including those involved in the metabolisms of cell wall, carbohydrates and antioxidants, light reactions, biotic and abiotic stress responses, as well as genes coding for transcription factors (TFs), protein kinases (PKs) and proteins involved in the abscisic acid (ABA) and ethylene signaling pathways, were differentially regulated by drought stress. RNA-Seq data were validated by quantitative real-time PCR (qPCR) analysis and comparative analysis of expression of the selected genes between sweet orange grafted on drought-tolerant and -sensitive rootstocks revealed new candidate genes for drought tolerance in citrus. CONCLUSIONS: In conclusion, our results showed that only a relatively small but functionally diverse fraction of the sweet orange transcriptome, with functions in metabolism, cellular responses and regulation, was differentially regulated by drought stress. The data suggest that the rootstock-induced drought tolerance in sweet orange includes the transcriptional activation of genes related to the cell wall, soluble carbohydrate and antioxidant metabolisms, biotic and abiotic stress responses, TFs, PKs and ABA signaling pathway, and the downregulation of genes involved in the starch metabolism, light reactions and ethylene signaling. Future efforts to elucidate their functional roles and explore their potential in the citrus genetic improvement should benefit from this data. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5481-z) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-06 /pmc/articles/PMC6364419/ /pubmed/30727949 http://dx.doi.org/10.1186/s12864-019-5481-z Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Gonçalves, Luana P. Boscariol Camargo, Raquel L. Takita, Marco Aurélio Machado, Marcos A. dos Soares Filho, Walter S. Costa, Marcio G. C. Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq |
title | Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq |
title_full | Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq |
title_fullStr | Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq |
title_full_unstemmed | Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq |
title_short | Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq |
title_sort | rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by rna-seq |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364419/ https://www.ncbi.nlm.nih.gov/pubmed/30727949 http://dx.doi.org/10.1186/s12864-019-5481-z |
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