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Outer hair cells isolation from postnatal Sprague–Dawley rats
Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basila...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364511/ https://www.ncbi.nlm.nih.gov/pubmed/30775696 http://dx.doi.org/10.1016/j.wjorl.2018.01.001 |
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author | Qi, Mei-Hao Qiu, Yang Tian, Ke-Yong Liang, Kun Chang, Hui-Min Wang, Ren-Feng Chen, Er-Fang Wang, Wei-Long Zha, Ding-Jun Qiu, Jian-Hua |
author_facet | Qi, Mei-Hao Qiu, Yang Tian, Ke-Yong Liang, Kun Chang, Hui-Min Wang, Ren-Feng Chen, Er-Fang Wang, Wei-Long Zha, Ding-Jun Qiu, Jian-Hua |
author_sort | Qi, Mei-Hao |
collection | PubMed |
description | Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basilar membrane which contains massive cells with different types. Therefore, to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing. In the present study, we tried to isolate OHCs from the commonly used animal model –Sprague-Dawley (SD) rats. By separating outer hair cells from SD rats with different day ages, we found that 9 days after birth was a suitable time for the separation of the OHCs. At this time, the number of OHCs isolated from rats was large, and the cell morphology was typical of cylindrical shape. OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses. |
format | Online Article Text |
id | pubmed-6364511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-63645112019-02-15 Outer hair cells isolation from postnatal Sprague–Dawley rats Qi, Mei-Hao Qiu, Yang Tian, Ke-Yong Liang, Kun Chang, Hui-Min Wang, Ren-Feng Chen, Er-Fang Wang, Wei-Long Zha, Ding-Jun Qiu, Jian-Hua World J Otorhinolaryngol Head Neck Surg Research Paper Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basilar membrane which contains massive cells with different types. Therefore, to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing. In the present study, we tried to isolate OHCs from the commonly used animal model –Sprague-Dawley (SD) rats. By separating outer hair cells from SD rats with different day ages, we found that 9 days after birth was a suitable time for the separation of the OHCs. At this time, the number of OHCs isolated from rats was large, and the cell morphology was typical of cylindrical shape. OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses. KeAi Publishing 2019-01-11 /pmc/articles/PMC6364511/ /pubmed/30775696 http://dx.doi.org/10.1016/j.wjorl.2018.01.001 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Qi, Mei-Hao Qiu, Yang Tian, Ke-Yong Liang, Kun Chang, Hui-Min Wang, Ren-Feng Chen, Er-Fang Wang, Wei-Long Zha, Ding-Jun Qiu, Jian-Hua Outer hair cells isolation from postnatal Sprague–Dawley rats |
title | Outer hair cells isolation from postnatal Sprague–Dawley rats |
title_full | Outer hair cells isolation from postnatal Sprague–Dawley rats |
title_fullStr | Outer hair cells isolation from postnatal Sprague–Dawley rats |
title_full_unstemmed | Outer hair cells isolation from postnatal Sprague–Dawley rats |
title_short | Outer hair cells isolation from postnatal Sprague–Dawley rats |
title_sort | outer hair cells isolation from postnatal sprague–dawley rats |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364511/ https://www.ncbi.nlm.nih.gov/pubmed/30775696 http://dx.doi.org/10.1016/j.wjorl.2018.01.001 |
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