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Outer hair cells isolation from postnatal Sprague–Dawley rats

Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basila...

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Autores principales: Qi, Mei-Hao, Qiu, Yang, Tian, Ke-Yong, Liang, Kun, Chang, Hui-Min, Wang, Ren-Feng, Chen, Er-Fang, Wang, Wei-Long, Zha, Ding-Jun, Qiu, Jian-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364511/
https://www.ncbi.nlm.nih.gov/pubmed/30775696
http://dx.doi.org/10.1016/j.wjorl.2018.01.001
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author Qi, Mei-Hao
Qiu, Yang
Tian, Ke-Yong
Liang, Kun
Chang, Hui-Min
Wang, Ren-Feng
Chen, Er-Fang
Wang, Wei-Long
Zha, Ding-Jun
Qiu, Jian-Hua
author_facet Qi, Mei-Hao
Qiu, Yang
Tian, Ke-Yong
Liang, Kun
Chang, Hui-Min
Wang, Ren-Feng
Chen, Er-Fang
Wang, Wei-Long
Zha, Ding-Jun
Qiu, Jian-Hua
author_sort Qi, Mei-Hao
collection PubMed
description Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basilar membrane which contains massive cells with different types. Therefore, to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing. In the present study, we tried to isolate OHCs from the commonly used animal model –Sprague-Dawley (SD) rats. By separating outer hair cells from SD rats with different day ages, we found that 9 days after birth was a suitable time for the separation of the OHCs. At this time, the number of OHCs isolated from rats was large, and the cell morphology was typical of cylindrical shape. OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses.
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spelling pubmed-63645112019-02-15 Outer hair cells isolation from postnatal Sprague–Dawley rats Qi, Mei-Hao Qiu, Yang Tian, Ke-Yong Liang, Kun Chang, Hui-Min Wang, Ren-Feng Chen, Er-Fang Wang, Wei-Long Zha, Ding-Jun Qiu, Jian-Hua World J Otorhinolaryngol Head Neck Surg Research Paper Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basilar membrane which contains massive cells with different types. Therefore, to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing. In the present study, we tried to isolate OHCs from the commonly used animal model –Sprague-Dawley (SD) rats. By separating outer hair cells from SD rats with different day ages, we found that 9 days after birth was a suitable time for the separation of the OHCs. At this time, the number of OHCs isolated from rats was large, and the cell morphology was typical of cylindrical shape. OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses. KeAi Publishing 2019-01-11 /pmc/articles/PMC6364511/ /pubmed/30775696 http://dx.doi.org/10.1016/j.wjorl.2018.01.001 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Qi, Mei-Hao
Qiu, Yang
Tian, Ke-Yong
Liang, Kun
Chang, Hui-Min
Wang, Ren-Feng
Chen, Er-Fang
Wang, Wei-Long
Zha, Ding-Jun
Qiu, Jian-Hua
Outer hair cells isolation from postnatal Sprague–Dawley rats
title Outer hair cells isolation from postnatal Sprague–Dawley rats
title_full Outer hair cells isolation from postnatal Sprague–Dawley rats
title_fullStr Outer hair cells isolation from postnatal Sprague–Dawley rats
title_full_unstemmed Outer hair cells isolation from postnatal Sprague–Dawley rats
title_short Outer hair cells isolation from postnatal Sprague–Dawley rats
title_sort outer hair cells isolation from postnatal sprague–dawley rats
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364511/
https://www.ncbi.nlm.nih.gov/pubmed/30775696
http://dx.doi.org/10.1016/j.wjorl.2018.01.001
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