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The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients

OBJECTIVE: To identify the differences in cytokine expression between sinonasal tissue from patients treated with Leptospermum (Manuka) honey (LH) irrigation versus normal saline irrigation twice-daily for twelve weeks following sinus surgery (FESS). METHODS: Forty-six CRS patients were recruited. S...

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Autores principales: Manji, Jamil, Thamboo, Andrew, Sunkaraneni, Vishnu, Singh, Amrit, Tebbutt, Scott, Garnis, Cathie, Javer, Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364513/
https://www.ncbi.nlm.nih.gov/pubmed/30775697
http://dx.doi.org/10.1016/j.wjorl.2018.07.004
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author Manji, Jamil
Thamboo, Andrew
Sunkaraneni, Vishnu
Singh, Amrit
Tebbutt, Scott
Garnis, Cathie
Javer, Amin
author_facet Manji, Jamil
Thamboo, Andrew
Sunkaraneni, Vishnu
Singh, Amrit
Tebbutt, Scott
Garnis, Cathie
Javer, Amin
author_sort Manji, Jamil
collection PubMed
description OBJECTIVE: To identify the differences in cytokine expression between sinonasal tissue from patients treated with Leptospermum (Manuka) honey (LH) irrigation versus normal saline irrigation twice-daily for twelve weeks following sinus surgery (FESS). METHODS: Forty-six CRS patients were recruited. Sinus tissue biopsies were collected during FESS and then at 5 and 12 weeks postoperatively during the course of treatment. A multi-plex cytokine assay quantified the abundance of 17 cytokines in biopsied tissue. Cytokine expression fold-change was analyzed between each time point using a robust linear regression model and compared between the two treatment groups. RESULTS: Compared to the saline irrigation group, five cytokines were differently expressed (CI = 95%) in sinonasal tissue obtained from subjects in the LH irrigation group during the 12-week treatment period. Cytokines IL-6 (P = 0.0400), IL-8 (P = 0.0398), MCP-1 (P = 0.0284), and MIP-1β (P = 0.016) were significantly increased in the LH irrigation group compared to the saline irrigation group. IL-13 was significantly increased in the saline irrigation group compared to the LH group (P = 0.0086). CONCLUSION: LH may potentially act to modulate the expression of IL-6, IL-8, IL-13, MCP-1 and MIP-1β in sinonasal tissue.
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spelling pubmed-63645132019-02-15 The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients Manji, Jamil Thamboo, Andrew Sunkaraneni, Vishnu Singh, Amrit Tebbutt, Scott Garnis, Cathie Javer, Amin World J Otorhinolaryngol Head Neck Surg Research Paper OBJECTIVE: To identify the differences in cytokine expression between sinonasal tissue from patients treated with Leptospermum (Manuka) honey (LH) irrigation versus normal saline irrigation twice-daily for twelve weeks following sinus surgery (FESS). METHODS: Forty-six CRS patients were recruited. Sinus tissue biopsies were collected during FESS and then at 5 and 12 weeks postoperatively during the course of treatment. A multi-plex cytokine assay quantified the abundance of 17 cytokines in biopsied tissue. Cytokine expression fold-change was analyzed between each time point using a robust linear regression model and compared between the two treatment groups. RESULTS: Compared to the saline irrigation group, five cytokines were differently expressed (CI = 95%) in sinonasal tissue obtained from subjects in the LH irrigation group during the 12-week treatment period. Cytokines IL-6 (P = 0.0400), IL-8 (P = 0.0398), MCP-1 (P = 0.0284), and MIP-1β (P = 0.016) were significantly increased in the LH irrigation group compared to the saline irrigation group. IL-13 was significantly increased in the saline irrigation group compared to the LH group (P = 0.0086). CONCLUSION: LH may potentially act to modulate the expression of IL-6, IL-8, IL-13, MCP-1 and MIP-1β in sinonasal tissue. KeAi Publishing 2018-09-07 /pmc/articles/PMC6364513/ /pubmed/30775697 http://dx.doi.org/10.1016/j.wjorl.2018.07.004 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Manji, Jamil
Thamboo, Andrew
Sunkaraneni, Vishnu
Singh, Amrit
Tebbutt, Scott
Garnis, Cathie
Javer, Amin
The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients
title The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients
title_full The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients
title_fullStr The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients
title_full_unstemmed The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients
title_short The association of Leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients
title_sort association of leptospermum honey with cytokine expression in the sinonasal epithelium of chronic rhinosinusitis patients
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364513/
https://www.ncbi.nlm.nih.gov/pubmed/30775697
http://dx.doi.org/10.1016/j.wjorl.2018.07.004
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