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lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3
Considering the joint contribution of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs) to tumorigenesis, the aim of the present study was to investigate whether and how lncRNA H19 targets miR-29b-3p to affect the progression of lung adenocarcinoma by the modulation of signal transducer and...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365046/ https://www.ncbi.nlm.nih.gov/pubmed/30747209 http://dx.doi.org/10.3892/ijo.2019.4695 |
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author | Liu, Lihua Liu, Linlin Lu, Sijing |
author_facet | Liu, Lihua Liu, Linlin Lu, Sijing |
author_sort | Liu, Lihua |
collection | PubMed |
description | Considering the joint contribution of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs) to tumorigenesis, the aim of the present study was to investigate whether and how lncRNA H19 targets miR-29b-3p to affect the progression of lung adenocarcinoma by the modulation of signal transducer and activator of transcription 3 (STAT3). A total of 305 lung adenocarcinoma tissues and four human lung adenocarcinoma cell lines (i.e. Calu-3, NCI-H1975, A549 and NCI-H23) were used. pcDNA3.1-H19, short interfering RNA (si-)H19, miR-29b-3p mimic, miR-29b-3p inhibitor and negative control (NC) were transfected into the cells, and the proliferation, viability and apoptosis of the cells were determined using a Cell Counting Kit-8 assay, colony formation assay and flow cytometry, respectively. The results indicated that highly expressed H19 and poorly expressed miR-29b-3p could serve as predictors for the poor prognosis of lung adenocarcinoma patients. Additionally, si-H19 and miR-29b-3p mimic significantly increased the apoptosis of lung adenocarcinoma cells, and decreased the survival rate and viability of cells. Simultaneously, expression of epithelial-mesenchymal transition (EMT) -specific proteins was significantly altered, i.e. increased epithelial cadherin expression, as well as decreased vimentin, Snail and Slug expression. Furthermore, miR-29b-3p was verified to be targeted and regulated by H19, and STAT3 was targeted and modified by miR-29b-3p. Ultimately, STAT3 was identified to decrease lung adenocarcinoma cell viability, survival, apoptosis and EMT imposed by miR-29b-3p. In conclusion, the results of the present study indicated that lncRNA H19/miR-29b-3p/STAT3 signaling was involved in the development of lung adenocarcinoma, which may be critical for developing effective diagnostic and treatment strategies for lung adenocarcinoma. |
format | Online Article Text |
id | pubmed-6365046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63650462019-02-19 lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3 Liu, Lihua Liu, Linlin Lu, Sijing Int J Oncol Articles Considering the joint contribution of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs) to tumorigenesis, the aim of the present study was to investigate whether and how lncRNA H19 targets miR-29b-3p to affect the progression of lung adenocarcinoma by the modulation of signal transducer and activator of transcription 3 (STAT3). A total of 305 lung adenocarcinoma tissues and four human lung adenocarcinoma cell lines (i.e. Calu-3, NCI-H1975, A549 and NCI-H23) were used. pcDNA3.1-H19, short interfering RNA (si-)H19, miR-29b-3p mimic, miR-29b-3p inhibitor and negative control (NC) were transfected into the cells, and the proliferation, viability and apoptosis of the cells were determined using a Cell Counting Kit-8 assay, colony formation assay and flow cytometry, respectively. The results indicated that highly expressed H19 and poorly expressed miR-29b-3p could serve as predictors for the poor prognosis of lung adenocarcinoma patients. Additionally, si-H19 and miR-29b-3p mimic significantly increased the apoptosis of lung adenocarcinoma cells, and decreased the survival rate and viability of cells. Simultaneously, expression of epithelial-mesenchymal transition (EMT) -specific proteins was significantly altered, i.e. increased epithelial cadherin expression, as well as decreased vimentin, Snail and Slug expression. Furthermore, miR-29b-3p was verified to be targeted and regulated by H19, and STAT3 was targeted and modified by miR-29b-3p. Ultimately, STAT3 was identified to decrease lung adenocarcinoma cell viability, survival, apoptosis and EMT imposed by miR-29b-3p. In conclusion, the results of the present study indicated that lncRNA H19/miR-29b-3p/STAT3 signaling was involved in the development of lung adenocarcinoma, which may be critical for developing effective diagnostic and treatment strategies for lung adenocarcinoma. D.A. Spandidos 2019-01-24 /pmc/articles/PMC6365046/ /pubmed/30747209 http://dx.doi.org/10.3892/ijo.2019.4695 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Lihua Liu, Linlin Lu, Sijing lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3 |
title | lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3 |
title_full | lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3 |
title_fullStr | lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3 |
title_full_unstemmed | lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3 |
title_short | lncRNA H19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting miR-29b-3p and modifying STAT3 |
title_sort | lncrna h19 promotes viability and epithelial-mesenchymal transition of lung adenocarcinoma cells by targeting mir-29b-3p and modifying stat3 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365046/ https://www.ncbi.nlm.nih.gov/pubmed/30747209 http://dx.doi.org/10.3892/ijo.2019.4695 |
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