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Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans

Adaptive responses to external temperatures are essential for survival in changing environments. We show here that environmental oxygen concentration affects cold acclimation in Caenorhabditis elegans and that this response is regulated by a KCNQ-type potassium channel, KQT-2. Depending on culture c...

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Autores principales: Okahata, Misaki, Wei, Aguan D., Ohta, Akane, Kuhara, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365114/
https://www.ncbi.nlm.nih.gov/pubmed/30775442
http://dx.doi.org/10.1126/sciadv.aav3631
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author Okahata, Misaki
Wei, Aguan D.
Ohta, Akane
Kuhara, Atsushi
author_facet Okahata, Misaki
Wei, Aguan D.
Ohta, Akane
Kuhara, Atsushi
author_sort Okahata, Misaki
collection PubMed
description Adaptive responses to external temperatures are essential for survival in changing environments. We show here that environmental oxygen concentration affects cold acclimation in Caenorhabditis elegans and that this response is regulated by a KCNQ-type potassium channel, KQT-2. Depending on culture conditions, kqt-2 mutants showed supranormal cold acclimation, caused by abnormal thermosensation in ADL chemosensory neurons. ADL neurons are responsive to temperature via transient receptor potential channels—OSM-9, OCR-2, and OCR-1—with OCR-1 negatively regulating ADL function. Similarly, KQT-2 and KQT-3 regulate ADL activity, with KQT-2 positively regulating ADL function. Abnormal cold acclimation and acute temperature responses of ADL neurons in kqt-2 mutants were suppressed by an oxygen-receptor mutation in URX coelomic sensory neurons, which are electrically connected to ADL via RMG interneurons. Likewise, low oxygen suppressed supranormal kqt-2 cold acclimation. These data thus demonstrate a simple neuronal circuit integrating two different sensory modalities, temperature and oxygen, that determines cold acclimation.
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spelling pubmed-63651142019-02-15 Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans Okahata, Misaki Wei, Aguan D. Ohta, Akane Kuhara, Atsushi Sci Adv Research Articles Adaptive responses to external temperatures are essential for survival in changing environments. We show here that environmental oxygen concentration affects cold acclimation in Caenorhabditis elegans and that this response is regulated by a KCNQ-type potassium channel, KQT-2. Depending on culture conditions, kqt-2 mutants showed supranormal cold acclimation, caused by abnormal thermosensation in ADL chemosensory neurons. ADL neurons are responsive to temperature via transient receptor potential channels—OSM-9, OCR-2, and OCR-1—with OCR-1 negatively regulating ADL function. Similarly, KQT-2 and KQT-3 regulate ADL activity, with KQT-2 positively regulating ADL function. Abnormal cold acclimation and acute temperature responses of ADL neurons in kqt-2 mutants were suppressed by an oxygen-receptor mutation in URX coelomic sensory neurons, which are electrically connected to ADL via RMG interneurons. Likewise, low oxygen suppressed supranormal kqt-2 cold acclimation. These data thus demonstrate a simple neuronal circuit integrating two different sensory modalities, temperature and oxygen, that determines cold acclimation. American Association for the Advancement of Science 2019-02-06 /pmc/articles/PMC6365114/ /pubmed/30775442 http://dx.doi.org/10.1126/sciadv.aav3631 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Okahata, Misaki
Wei, Aguan D.
Ohta, Akane
Kuhara, Atsushi
Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans
title Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans
title_full Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans
title_fullStr Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans
title_full_unstemmed Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans
title_short Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans
title_sort cold acclimation via the kqt-2 potassium channel is modulated by oxygen in caenorhabditis elegans
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365114/
https://www.ncbi.nlm.nih.gov/pubmed/30775442
http://dx.doi.org/10.1126/sciadv.aav3631
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